소장자료

Cereal genomics: bethods and protocols [electronic resource]

  • Vaschetto, Luis M.
  • Humana Press
  • 2020
Cereal genomics: bethods and protocols [electronic resource]
  • 자료유형
    단행본
  • 서명/저자사항
    Cereal genomics:bethods and protocols[electronic resource] / edited by Luis M. Vaschetto.
  • 발행사항
    Totowa, NJ : Humana Press, 2020.
  • 개인저자
    Vaschetto, Luis M.
  • 형태사항
    1 online resource.
  • 총서명
    Methods in Molecular Biology ; 2072
  • 일반주제명
    Plant genetics
  • ISBN
    9781493998647, 9781493998654
  • 언어
    영어

초록

The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

목차

A Low-Cost High-Throughput Method for Plant Genomic DNA Isolation / Prateek Gupta, Hymavathi Salava, Yellamaraju Sreelakshmi, Rameshwar Sharma
Cereal Genomics Databases and Plant Genetic Resources in Crop Improvement / Robert J. Henry
Integrated Genomic Strategies for Cereal Genetic Enhancement: Combining QTL and Association Mapping / Anurag Daware, Swarup K. Parida, Akhilesh K. Tyagi
Sequencing and Assembling Genomes and Chromosomes of Cereal Crops / Marcelo Helguera
Cost-Effective Profiling of Mutator Transposon Insertions in Maize by Next-Generation Sequencing / Xinyan Zhang, Meixia Zhao, Damon Lisch
Relative Expression Analysis of Target Genes by Using Reverse Transcription-Quantitative PCR / Rocío Liliana Gómez, Lorena Noelia Sendín
DNA Methylation and Transcriptomic Next-Generation Technologies in Cereal Genomics / Cynthia G. Soto-Cardinault, Fátima Duarte-Aké, Clelia De-la-Peña, Elsa Góngora-Castillo
Genome-Wide Identification of Regulatory DNA Elements in Crop Plants / Zhanjie Li, Kai Wang
Genome-Wide Profiling of Histone Modifications with ChIP-Seq / William A. Ricci, Laura Levin, Xiaoyu Zhang
Whole-Genome Bisulfite Sequencing and Epigenetic Variation in Cereal Methylomes / Taiji Kawakatsu
Genome-Wide Identification of Allele-Specific Gene Expression in a Parent-of-Origin Specific Manner / Chen Chen, Kevin Begcy
Analysis of Epigenetic Modifications During Vegetative and Reproductive Development in Cereals Using Chromatin Immunoprecipitation (ChIP) / Kevin Begcy, Thomas Dresselhaus
Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis / Luis M. Vaschetto, Celso Gaspar Litholdo Jr, Lorena Noelia Sendín, Claudia Mabel Terenti Romero, María Paula Filippone
Emerging Genome Engineering Tools in Crop Research and Breeding / Andriy Bilichak, Daniel Gaudet, John Laurie
CRISPR/Cas9-Mediated Targeted Mutagenesis in Wheat Doubled Haploids / Alison M. R. Ferrie, Pankaj Bhowmik, Nandhakishore Rajagopalan, Sateesh Kagale
Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne / Huw R. Davis, Anne L. Maddison, Dylan W. Phillips, Huw D. Jones
Agrobacterium Transformation in the Rice Genome / Asuka Nishimura
In Vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C4 Photosynthesis in Zea mays / Yoshihisa Ueno, Kumiko Yoshizawa-Kumagaye, Junji Emura, Tomoko Urabe, Taku Yoshiya, Tsuyoshi Furumoto et al.