소장자료

Adrenergic Receptor Protocols [electronic resource]

  • Machida, Curtis A. editor.
  • 2000
Adrenergic Receptor Protocols [electronic resource]
  • 자료유형
    단행본
  • 서명/저자사항
    Adrenergic Receptor Protocols[electronic resource] / edited by Curtis A. Machida.
  • 개인저자
    Machida, Curtis A., editor.
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    XIV, 577 p. : online resource.
  • 총서명
    Methods in Molecular Biology,1064-3745 ; 126
  • 내용주기
    Genetic Analysis -- Construction of Libraries for Isolation of Adrenergic Receptor Genes -- Isolation of Adrenergic Receptor Genes -- Analyses of Adrenergic Receptor Sequences -- Polymerase Chain Reaction Screening of Genomic Libraries for Adrenergic Receptor Genes -- Solution-Phase Library Screening for Identification of Rare Adrenergic Receptor Clones -- Genetic Polymorphisms of Adrenergic Receptors -- RNA Analysis -- Northern Blot Analyses Detecting Adrenergic Receptor mRNAs -- Ribonuclease Protection Assay for the Detection of ?1-Adrenergic Receptor RNA -- Determination of Adrenergic Receptor mRNAs by Quantitative Reverse Transcriptase-Polymerase Chain Reactions -- Nuclear Run-On Assays for Measurement of Adrenergic Receptor Transcription Rate -- Primer Extension Methods for Determination of ?1-Adrenergic Receptor mRNA Start Sites -- Expression Analysis -- Use of Eukaryotic Vectors for the Expression of Adrenergic Receptors -- Expression of ?-Adrenergic Receptors in Recombinant Baculovirus-Infected Insect Cells -- Expression of ?-Adrenergic Receptors in E. coli -- Use and Pharmacological Analysis of Established and Transfected Cell Lines Expressing Adrenergic Receptors -- Transient Transfection and Adrenergic Receptor Promoter Analysis -- Antisense RNA/DNA-Based Techniques to Probe Adrenergic Receptor Function -- Targeted Disruption of Adrenergic Receptor Genes -- Receptor and Second Messenger Analysis -- Development of Antibodies to Adrenergic Receptors -- Western Blot Detection of Adrenergic Receptors -- UV Crosslinking of Adrenergic Receptors and Ligands -- Detection of ?-Adrenergic Receptors by Radioligand Binding -- Assessing Adrenergic Receptor Conformation Using Chemically Reactive Fluorescent Probes -- Biochemical Methods for Detection and Measurement of Cyclic AMP and Adenylyl Cyclase Activity -- Assay of Arachidonic Acid Release Coupled to ?1- and ?2-Adrenergic Receptors -- Patch-Clamp Recording Methods for Examining Adrenergic Regulation of Potassium Currents in Ocular Epithelial Cells -- Transactivator Analysis -- Southwestern Blots for Detection of a DNA Binding Protein Recognizing the ?1B-Adrenergic Receptor Gene Promoter -- DNase I Footprinting Analysis of Transcription Factors Recognizing Adrenergic Receptor Gene Promoter Sequences -- Electrophoretic Mobility Shift Assay for Detection of DNA Binding Proteins Recognizing ?-Adrenergic Receptor Gene Sequences -- Determination of mRNA Stability and Characterization of Proteins Interacting with Adrenergic Receptor mRNAs -- Microscopy Analysis -- Use of Immunohistochemistry and Confocal Microscopy in the Detection of Adrenergic Receptors -- Distribution of ?2A- and ?2C-Adrenergic Receptor Immunoreactivity in the Central Nervous System -- Quantitative Light Microscopic Autoradiography of ?2-Adrenergic Radioligand Binding Sites -- In Situ Hybridization of Adrenergic Receptor mRNA in Brain -- Use of Electron Microscopy in the Detection of Adrenergic Receptors.
  • 일반주제명
    Medicine.
    Neurosciences.
    Biomedicine.
    Neurosciences.
  • 기본자료 저록
    Springer eBooks
  • 기타형태 저록
    Printed edition: 9780896036024
  • ISBN
    9781592596843
  • 언어
    영어