소장자료

Retinal Degeneration: Methods and Protocols [electronic resource]

  • Weber, Bernhard H. F. editor. , Langmann, Thomas. editor.
  • Springer
  • 2019
Retinal Degeneration: Methods and Protocols [electronic resource]
  • 자료유형
    단행본
  • 서명/저자사항
    Retinal Degeneration:Methods and Protocols[electronic resource] / edited by Bernhard H. F. Weber, Thomas Langmann.
  • 판차
    2nd ed. 2019.
  • 발행사항
    New York, NY : Springer, 2019.
  • 개인저자
    Weber, Bernhard H. F., editor., Langmann, Thomas., editor.
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    XIV, 417 p. 108 illus., 56 illus. in color. : online resource.
  • 총서명
    Methods in Molecular Biology,1064-3745 ; 1834
  • 내용주기
    Identification and Analysis of Genes Associated with Inherited Retinal Diseases -- eQTL Analysis in Retinal Research -- Testing for known Retinal Degeneration Mutants in Mouse Strains -- CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo -- Monitoring Surface Reactions by Combined Western Blot-ELISA Analysis -- Generation of Functional Retinal Pigment Epithelium from Human Induced Pluripotent Stem Cells -- Advanced Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture -- Porcine RPE/Choroidal Explant Cultures -- The Mouse Retinal Organoid Trisection Recipe: Efficient Generation of 3D Retinal Tissue from Mouse Embryonic Stem Cells -- Cell Death Analysis in Retinal Cultures -- Fate Mapping In Vivo to Distinguish Bona Fide Microglia Versus Recruited Monocyte-derived Macrophages in Retinal Disease -- Light Damage Models of Retinal Degeneration -- Induction and Readout of Oxygen-Iinduced Retinopathy -- Generation and Analysis of Xenopus laevis Models of Retinal Degeneration using CRISPR/Cas9 -- Gene Knockdown in Zebrafish (Danio rerio) as a Tool to Model Photoreceptor Diseases -- Drosophila melanogaster - A Valuable Genetic Model Organism to Elucidate the Biology of Retinitis Pigmentosa -- Retinal Fundus Imaging in Mouse Models of Retinal Diseases -- Phenotyping of Mouse Models with OCT -- Cell-specific Markers for the Identification of Retinal Cells and Subcellular Organelles by Immunofluorescence Microscopy -- Immuno-TEM/STEM in Retinal Research -- Noninvasive Two-photon Microscopy Imaging of Mouse Retina and Retinal Pigment Epithelium -- Cell-based Therapy for Retinal Disease: The New Frontier -- In Vitro Evaluation of AAV Vectors for Retinal Gene Therapy -- Nanoparticles Targeting Retinal and Choroidal Capillaries In Vivo -- Optimized Subretinal Injection Technique for Gene Therapy Approaches.
  • 일반주제명
    Medicine.
    Molecular biology.
    Biomedicine.
    Molecular Medicine.
  • 기본자료 저록
    Springer eBooks
  • 기타형태 저록
    Printed edition: 9781493986682
  • ISBN
    9781493986699
  • 언어
    영어

초록

This volume provides key updates on several of the first edition chapters and also includes new novel techniques, addressing the most recent technological developments and their applications in retinal research. Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.