소장자료

Brain Tumor Stem Cells: Methods and Protocols [electronic resource]

  • Singh, Sheila K. editor. , Venugopal, Chitra. editor.
  • Springer
  • 2019
Brain Tumor Stem Cells: Methods and Protocols [electronic resource]
  • 자료유형
    단행본
  • 서명/저자사항
    Brain Tumor Stem Cells:Methods and Protocols[electronic resource] / edited by Sheila K. Singh, Chitra Venugopal.
  • 발행사항
    New York, NY : Springer, 2019.
  • 개인저자
    Singh, Sheila K., editor., Venugopal, Chitra., editor.
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    XII, 254 p. 40 illus., 34 illus. in color. : online resource.
  • 총서명
    Methods in Molecular Biology,1064-3745 ; 1869
  • 내용주기
    Introduction to Brain Tumor Stem Cells -- Isolation and Culture of Glioblastoma Brain Tumor Stem Cells -- Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines -- Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors -- Detecting Stem Cell Marker Expression Using the NanoString nCounter System -- Flow Cytometric Analysis of Brain Tumor Stem Cells -- In Vitro Self-Renewal Assays for Brain Tumor Stem Cells -- Differentiation of Brain Tumor Initiating Cells -- The Study of Brain Tumor Stem Cell Migration -- The Study of Brain Tumor Stem Cell Invasion -- Cell Cycle Dynamics in Glioma Cancer Stem Cells -- Embryonic Stem Cell Models of Human Brain Tumors -- Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory -- EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF -- Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells -- In Vitro Assays for Screening Small Molecules -- Drug Delivery in an Orthotopic Tumor Stem Cell-Based Model of Human Glioblastoma -- Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci -- In Vivo Murine Models of Brain Metastasis -- Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain.
  • 일반주제명
    Medicine.
    Cancer research.
    Neurosciences.
    Biomedicine.
    Cancer Research.
    Neurosciences.
  • 기본자료 저록
    Springer eBooks
  • 기타형태 저록
    Printed edition: 9781493988044
  • ISBN
    9781493988051
  • 언어
    영어

초록

This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse. The BTSC model recapitulates scientific observations made in brain cancer patients, and these chapters provide the reader with a comprehensive understanding of the skills and techniques that will unlock data from this most informative subset of cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.