소장자료

Legionella : methods and protocols [electronic resource]

  • Buchrieser, Carmen , Hilbi, Hubert
  • Springer
  • 2019
Legionella : methods and protocols [electronic resource]

초록

The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila. Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, biochemical assays to study legionella effectors and enzymes, immunity and host response against legionella, metagenomics, proteomics, and host microbiomes of legionella. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Legionella: Methods and Protocols, Second Edition aims to be useful for scientists studying Legionella, and for a broader research community interested in the ecology, physiology, pathogenesis, immunity, genetics and evolution of other bacterial pathogens.

목차

Ecology and Physiology of Legionella
Analysis of Iron Requirements and Siderophore Production / Denise M. Burnside, Nicholas P. Cianciotto
The Pathometabolism of Legionella Studied by Isotopologue Profiling / Klaus Heuner, Mareike Kunze, Fan Chen, Wolfgang Eisenreich
Quantification of Viable but Non-Culturable Cells of Legionella pneumophila / Rosalie S. Braun, Nilmini Mendis, Laam Li, Sebastien P. Faucher
Selection of Legionella Virulence-Related Traits by Environmental Protozoa / Francisco Amaro, Howard Shuman
Migration of Acanthamoeba castellanii Through Legionella Biofilms / Ramon Hochstrasser, Hubert Hilbi

Legionella Genetics
Scar-Free Genome Editing in Legionella pneumophila / Nathalie Bailo, Hussein Kanaan, Elisabeth Kay, Xavier Charpentier, Patricia Doublet, Christophe Gilbert
Querying Legionella Genomes Using Transposition-Sequencing / Léo Hardy, Xavier Charpentier
Screening Targeted Legionella pneumophila Mutant Libraries In Vivo Using INSeq / Stephanie R. Shames
Genetic Manipulation of Non-pneumophila Legionella: Protocols Developed for Legionella longbeachae / Elizabeth Korevaar, Chen Ai Khoo, Hayley J. Newton

Cellular Microbiology of Legionella
Quantitative Imaging Flow Cytometry of Legionella-Containing Vacuoles in Dually Fluorescence-Labeled Dictyostelium / Amanda Welin, Stephen Weber, Hubert Hilbi
Sorting of Phagocytic Cells Infected with Legionella pneumophila / Monica Rolando, Carmen Buchrieser
Single Cell Analysis of Legionella and Legionella-Infected Acanthamoeba by Agarose Embedment / Nicolas Personnic, Bianca Striednig, Hubert Hilbi
Mitochondrial Dynamics and Activity in Legionella-Infected Cells / Ok-Ryul Song, Priscille Brodin, Carmen Buchrieser, Pedro Escoll
Perturbation of Legionella Cell Infection by RNA Interference / Bernhard Steiner, A. Leoni Swart, Hubert Hilbi

Biochemical Assays to Study Legionella Effectors and Enzymes
Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila / Tomoko Kubori, Hiroki Nagai
In Situ Imaging and Structure Determination of Bacterial Toxin Delivery Systems Using Electron Cryotomography / Debnath Ghosal, Mohammed Kaplan, Yi-Wei Chang, Grant J. Jensen
Methods for Noncanonical Ubiquitination and Deubiquitination Catalyzed by Legionella pneumophila Effector Proteins / Jiazhang Qiu, Zhao-Qing Luo
Purification and Analysis of Effector Glucosyltransferase Lgt1 from Legionella pneumophila / Nadezhda Levanova, Irina Tabakova, Thomas Jank, Yury Belyi
Determination of In Vivo Interactomes of Dot/Icm Type IV Secretion System Effectors by Tandem Affinity Purification / Ernest C. So, Aurélie Mousnier, Gad Frankel, Gunnar N. Schroeder
Inflammasome Activation in Legionella-Infected Macrophages / Danielle P. A. Mascarenhas, Dario S. Zamboni

Immunity and Host Response Against Legionella
Infection of Human Lung Tissue Explants (HLTEs) with Legionella pneumophila / Lina Scheithauer, Michael Steinert
The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella Virulence / Gad Frankel, Gunnar N. Schroeder
Dictyostelium Host Response to Legionella Infection: Strategies and Assays / Salvatore Bozzaro, Simona Buracco, Barbara Peracino, Ludwig Eichinger
The Caenorhabditis elegans Model of Legionella Infection / Ann Karen C. Brassinga, Costi D. Sifri
The Mouse as a Model for Pulmonary Legionella Infection / Garrett Z. Ng, Anastasia Solomatina, Ian R. van Driel, Elizabeth L. Hartland

Metagenomics, Proteomics, and Host Microbiomes of Legionella
Targeted Metagenomics of Microbial Diversity in Free-Living Amoebae and Water Samples / Vincent Delafont, Yoann Perrin, Didier Bouchon, Laurent Moulin, Yann Héchard
Analysis of the Pulmonary Microbiome Composition of Legionella pneumophila-Infected Patients / Ana Elena Pérez-Cobas, Carmen Buchrieser
Subcellular Protein Fractionation in Legionella pneumophila and Preparation of the Derived Sub-proteomes for Analysis by Mass Spectrometry / Sandra Maaß, Gina Moog, Dörte Becher