소장자료

Spliceosomal Pre-mRNA Splicing Methods and Protocols [electronic resource] :

  • Hertel, Klemens J. editor.
  • 2014
Spliceosomal Pre-mRNA Splicing Methods and Protocols [electronic resource] :
  • 자료유형
    단행본
  • 서명/저자사항
    Spliceosomal Pre-mRNA Splicing[electronic resource] :Methods and Protocols / edited by Klemens J. Hertel.
  • 개인저자
    Hertel, Klemens J., editor.
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    XI, 427 p. 66 illus., 34 illus. in color. : online resource.
  • 총서명
    Methods in Molecular Biology, Methods and Protocols,1064-3745 ; 1126
  • 내용주기
    The Pre-mRNA Splicing Reaction -- Diversity and Evolution of Spliceosomal Systems -- Mechanisms of Spliceosomal Assembly -- Alternative Pre-mRNA Splicing -- Regulation of Alternative Pre-mRNA Splicing -- Introduction to Co-Transcriptional RNA Splicing -- Chromatin and Splicing -- Preparation of Splicing Competent Nuclear Extracts -- Preparation of Yeast Whole Cell Splicing Extract -- Efficient Splinted Ligation of Synthetic RNA Using RNA Ligase -- In Vitro Assay of Pre-mRNA Splicing in Mammalian Nuclear Extract -- Kinetic Analysis of In Vitro Pre-mRNA Splicing in HeLa Nuclear Extract -- In Vitro Systems for Coupling RNAP II Transcription to Splicing and Polyadenylation -- Isolation and Accumulation of Spliceosomal Assembly Intermediates -- Complementation of U4 snRNA in S. cerevisiae Splicing Extracts for Biochemical Studies of snRNP Assembly and Function -- Expression and Purification of Splicing Proteins from Mammalian Cells -- Single Molecule Approaches for Studying Spliceosome Assembly and Catalysis -- Cell-Based Splicing of Minigenes -- Quantifying the Ratio of Spliceosome Components Assembled on Pre-mRNA -- Antisense Methods to Modulate Pre-mRNA Splicing -- Using Yeast Genetics to Study Splicing Mechanisms -- Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR -- Chromatin Immunoprecipitation Approaches to Determine Co-Transcriptional Nature of Splicing -- Computational Approaches to Mine Publicly Available Databases -- Approaches to Link RNA Secondary Structures with Splicing Regulation -- Methods to Study Splicing from High-Throughput RNA Sequencing Data -- Global Protein-RNA Interaction Mapping at Single Nucleotide Resolution by iCLIP-Seq -- Predicting Alternative Splicing.
  • 일반주제명
    Medicine.
    Human genetics.
    Biomedicine.
    Human Genetics.
  • 기본자료 저록
    Springer eBooks
  • 기타형태 저록
    Printed edition: 9781627039796
  • ISBN
    9781627039802
  • 언어
    영어