소장자료

RNA Methods and Protocols [electronic resource] :

  • Nielsen, Henrik. editor.
  • 2011
RNA Methods and Protocols [electronic resource] :
  • 자료유형
    단행본
  • 서명/저자사항
    RNA[electronic resource] :Methods and Protocols / edited by Henrik Nielsen.
  • 개인저자
    Nielsen, Henrik., editor.
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    XV, 329 p. 53 illus. : online resource.
  • 총서명
    Methods in Molecular Biology, Methods and Protocols,1064-3745 ; 703
  • 내용주기
    The Transcriptional Landscape -- Working with RNA -- Synthesis of RNA by In vitro Transcription -- Efficient Poly(A)+ RNA Selection Using LNA Oligo(T) Capture -- Genome Browsers -- Web-Based Tools for Studying RNA Structure and Function -- Northern Blotting Analysis -- Rapid Amplification of cDNA Ends (RACE) -- Fractionation of mRNA Based on the Length of the Poly(A) Tail -- Analysis of Mutations that Influence Pre-mRNA Splicing -- S1 Nuclease Analysis of Alternatively Spliced mRNA -- Promises and Challenges in Developing RNAi as a Research Tool and Therapy -- Inhibition of Gene Function in Mammalian Cells Using Short-Hairpin RNA (shRNA) -- Validation of RNAi by Real Time PCR -- Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method -- The Post-Transcriptional Operon -- RIP-Chip Analysis: RNA-Binding Protein Immunoprecipitation-Microarray (Chip) Profiling -- Isolation of RNP Granules -- Electrophoretic Mobility Shift Assay for Characterizing RNA-Protein Interaction -- Polysome Analysis and RNA Purification from Sucrose Gradients -- Prediction of Targets for microRNAs -- Outsourcing of Experimental Work -- ESM1: In-Line Probing Analysis of Riboswitches -- ESM2: Analysis of the RNA Backbone: Structural Analysis of Riboswitches by In-Line Probing and Selective 2'-Hydroxyl Acylation and Primer Extension (SHAPE).
  • 일반주제명
    Medicine.
    Human genetics.
    Gene expression.
    Biomedicine.
    Human Genetics.
    Gene Expression.
  • 기본자료 저록
    Springer eBooks
  • 기타형태 저록
    Printed edition: 9781588299130
  • ISBN
    9781597452489
  • 언어
    영어