소장자료

Biophysical Chemistry of Proteins An Introduction to Laboratory Methods [electronic resource] :

  • Buxbaum, Engelbert. author.
  • 2011
Biophysical Chemistry of Proteins An Introduction to Laboratory Methods [electronic resource] :
  • 자료유형
    단행본
  • 서명/저자사항
    Biophysical Chemistry of Proteins[electronic resource] :An Introduction to Laboratory Methods / by Engelbert Buxbaum.
  • 개인저자
    Buxbaum, Engelbert., author.
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    XVI, 510 p. : online resource.
  • 내용주기
    Analytical techniques. Microscopy. Single molecule techniques. Preparation of cells and tissues for microscopy. Principles of optical spectroscopy.Photometry. Fluorimetry. Chemiluminescence.Electrophoresis.Immunological methods.Isotope techniques -- Purification of proteins.Homogenisation and fractionisation of cells and tissues.Isolation of organelles. Precipitation methods.Chromatography. Membrane proteins. Determination of protein concentration.Cell culture -- Protein modification and inactivation. General technical remarks. Amine-reactive reagents. Thiol- and disulphide reactive reagents. Reagents for other groups. Cross-linkers.Detection methods. Spontaneous reactions in proteins -- Protein size and shape. Centrifugation.Osmotic pressure. Diffusion.Viscosity.Non-resonant interactions with electromagnetic waves -- Protein structure. Protein sequencing.Synthesis of peptides. Protein secondary structure. Structure of protein-ligand complexes. 3D-structures. Folding and unfolding of proteins -- Enzyme kinetics. Steady-state kinetics. Leaving the steady state: Analysis of progress curves.Reaction velocities. Isotope effects. Isotope exchange -- Protein-ligand interactions. General conditions for interpretable results. Binding equations.Methods to measure binding equilibria. Temperature effects on binding equilibrium and reaction rate -- Industrial enzymology. Industrial enzyme use.Immobilised enzymes -- Special statistics.Quality control. Testing whether or not a model fits the data -- Appendix. List of symbols.Greek alphabet. Properties of electrophoretic buffers. Bond properties. Acronyms.
  • 일반주제명
    Life sciences.
    Chemistry.
    Biochemistry.
    Cell biology.
    Biophysics.
    Biological physics.
    Life Sciences.
    Biochemistry, general.
    Cell Biology.
    Chemistry/Food Science, general.
    Biomedicine general.
    Biophysics and Biological Physics.
  • 기본자료 저록
    Springer eBooks
  • 기타형태 저록
    Printed edition: 9781441972507
  • ISBN
    9781441972514
  • 언어
    영어