소장자료

Cystic Fibrosis Diagnosis and Protocols, Volume I: Approaches to Study and Correct CFTR Defects [electronic resource] :

  • Amaral, Margarida D. editor. , Kunzelmann, Karl. editor.
  • 2011
Cystic Fibrosis Diagnosis and Protocols, Volume I: Approaches to Study and Correct CFTR Defects [electronic resource] :
  • 자료유형
    단행본
  • 서명/저자사항
    Cystic Fibrosis[electronic resource] :Diagnosis and Protocols, Volume I: Approaches to Study and Correct CFTR Defects / edited by Margarida D. Amaral, Karl Kunzelmann.
  • 개인저자
    Amaral, Margarida D., editor., Kunzelmann, Karl., editor.
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    XVI, 528 p. 76 illus., 4 illus. in color. : online resource.
  • 총서명
    Methods in Molecular Biology, Methods and Protocols,1064-3745 ; 741
  • 내용주기
    Introduction to Part I: The Relevance of CF Diagnostic Tools for Measuring Restoration of CFTR Function after Therapeutic Interventions in Human Clinical Trials -- High-Throughput Screening of Libraries of Compounds to Identify CFTR Modulators -- Repair of CFTR Folding Defects with Correctors that Function as Pharmacological Chaperones -- Use of Primary Cultures of Human Bronchial Epithelial Cells Isolated from Cystic Fibrosis Patients for the Pre-Clinical Testing of CFTR Modulators -- Design of Gene Therapy Trials in CF Patients -- Nasal Potential Difference Measurements to Assess CFTR Ion Channel Activity -- Measurement of Ion Transport Function in Rectal Biopsies -- Introduction to Part II: RNA Methods to Approach CFTR Expression -- Quantification of CFTR Transcripts -- Nonsense-Mediated mRNA Decay and Cystic Fibrosis -- Approaches to Study CFTR Pre-mRNA Splicing Defects -- Impact of MicroRNA in Normal and Pathological Respiratory Epithelia -- Genomic Approaches to Studying CFTR Transcriptional Regulation -- Introduction to Part III: Biochemical Methods to Study CFTR Protein -- Analysis of CFTR Folding and Degradation in Transiently Transfected Cells -- In Vitro Methods for CFTR Biogenesis -- Analysis of CFTR Interactome in the Macromolecular Complexes -- Methods to Monitor Cell Surface Expression and Endocytic Trafficking of CFTR in Polarized Epithelial Cells -- Segmental and Subcellular Distribution of CFTR in the Kidney -- Endocytic Sorting of CFTR Variants Monitored by Single Cell Fluorescence Ratio Image Analysis (FRIA) in Living Cells -- Introduction to Part IV: Biophysical Methods to Approach CFTR Structure -- CFTR Three-Dimensional Structure -- Molecular Modeling Tools and Approaches for CFTR and Cystic Fibrosis -- Biochemical and Biophysical Approaches to Probe CFTR Structure -- NMR Spectroscopy to Study the Dynamics and Interactions of CFTR -- Introduction to Part V: Assessment of CFTR Function -- Application of High-Resolution Single-Channel Recording to Functional Studies of Cystic Fibrosis Mutants -- Electrophysiological, Biochemical, and Bioinformatic Methods for Studying CFTR Channel Gating and Its Regulation -- CFTR Regulation by Phosphorylation -- How to Measure CFTR-Dependent Bicarbonate Transport: From Single Channels to the Intact Epithelium.
  • 일반주제명
    Medicine.
    Human genetics.
    Laboratory medicine.
    Molecular biology.
    Biomedicine.
    Molecular Medicine.
    Human Genetics.
    Laboratory Medicine.
  • 기본자료 저록
    Springer eBooks
  • 기타형태 저록
    Printed edition: 9781617791161
  • ISBN
    9781617791178
  • 언어
    영어