소장자료

Epigenome Editing Methods and Protocols [electronic resource] :

  • Jeltsch, Albert. editor. , Rots, Marianne G. editor.
  • 2018
Epigenome Editing Methods and Protocols [electronic resource] :
  • 자료유형
    단행본
  • 서명/저자사항
    Epigenome Editing[electronic resource] :Methods and Protocols / edited by Albert Jeltsch, Marianne G. Rots.
  • 개인저자
    Jeltsch, Albert., editor., Rots, Marianne G., editor.
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    XIV, 482 p. 78 illus., 66 illus. in color. : online resource.
  • 총서명
    Methods in Molecular Biology,1064-3745 ; 1767
  • 내용주기
    Editing the Epigenome: Overview, Open Questions, and Directions of Future Development -- Zinc Fingers, TALEs and CRISPR Systems: A Comparison of Tools for Epigenome Editing -- Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities -- Generation of TALE-Based Designer Epigenome Modifiers -- Neuroepigenetic Editing -- Allele-Specific Epigenome Editing -- Key to Delivery: The (Epi-)Genome Editing Vector Toolbox -- CRISPR/dCas9 Switch Systems for Temporal Transcriptional Control -- Delivery of Designer Epigenome Modifiers into Primary Human T Cells -- Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques -- Stable Expression of Epigenome Editors Via Viral Delivery and Genomic Integration -- Purified-Protein Delivery to Activate an Epigenetically-Silenced Allele in Mouse Brain -- Non-Viral Methodology for Efficient Co-Transfection -- Chromatin Immunoprecipitation in Human and Yeast Cells -- Chromatin Immunoprecipitation and High Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis -- Generation of Whole Genome Bisulfite Sequencing Libraries for Comprehensive DNA Methylome Analysis -- Approaches for the Analysis and Interpretation of Whole Genome Bisulfite Sequencing Data -- Whole Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution -- Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing -- DNA Methylation Analysis by Bisulfite Conversion Coupled to Double Multiplexed Amplicon-Based Next-Generation Sequencing (NGS) -- Cell-to-Cell Transcription Variability as Measured by Single Molecule RNA FISH to Detect Epigenetic State Switching -- Establishment of Cell Lines Stably Expressing dCas-Fusions to Address Kinetics of Epigenetic Editing -- Editing of DNA Methylation Using dCas9-Peptide Repeat and scFv-TET1 Catalytic Domain Fusions -- Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells -- Screening Regulatory Element Function with CRISPR/Cas9-Based Epigenome Editing.
  • 일반주제명
    Medicine.
    Human genetics.
    Biomedicine.
    Human Genetics.
  • 기본자료 저록
    Springer eBooks
  • 기타형태 저록
    Printed edition: 9781493977734
  • ISBN
    9781493977741
  • 언어
    영어