소장자료

Alpha-1 Antitrypsin Deficiency Methods and Protocols [electronic resource] :

  • Borel, Florie. editor. , Mueller, Christian. editor.
  • 2017
Alpha-1 Antitrypsin Deficiency Methods and Protocols [electronic resource] :
  • 자료유형
    단행본
  • 서명/저자사항
    Alpha-1 Antitrypsin Deficiency[electronic resource] :Methods and Protocols / edited by Florie Borel, Christian Mueller.
  • 개인저자
    Borel, Florie., editor., Mueller, Christian., editor.
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    XIII, 278 p. 71 illus., 42 illus. in color. : online resource.
  • 총서명
    Methods in Molecular Biology,1064-3745 ; 1639
  • 내용주기
    Pathophysiology of Alpha-1 Antitrypsin Deficiency Liver Disease -- Pathophysiology of Alpha-1 Antitrypsin Lung Disease -- Measuring and Interpreting Serum AAT Concentration -- AAT Phenotype Identification by Isoelectric Focusing -- Laboratory Diagnosis by Genotyping -- Genotyping Protocol for the Alpha-1 Antitrypsin (PiZ) Mouse Model -- Elastase-Induced Lung Emphysema Models in Mice -- Assessing Structure-Function Relations in Mice Using the Forced Oscillation Technique and Quantitative Histology -- Practical Methods for Assessing Emphysema Severity Based on Estimation of Linear Mean Intercept (Lm) in the Context of Animal Models of Alpha-1 Antitrypsin Deficiency -- Design, Cloning, and In Vitro Screening of Artificial miRNAs to Silence Alpha-1-Antitrypsin -- Methods to Identify and Characterize siRNAs Targeting Serpin A1 In Vitro and In Vivo using RNA Interference -- Knockdown of Z Mutant Alpha-1 Antitrypsin in Vivo Using Modified DNA Antisense Oligonucleotides -- Immunohistochemistry Staining for Human Alpha-1 Antitrypsin -- Periodic Acid-Schiff Staining with Diastase -- Protocol for Directed Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) to a Hepatic Lineage -- Isolation of Kupffer Cells and Hepatocytes from a Single Mouse Liver -- Alpha-1 Antitrypsin Transcytosis and Secretion -- Measuring the Effect of Histone Deacetylase Inhibitors (HDACi) on the Secretion and Activity of Alpha-1 Antitrypsin -- Expression and Purification of Active Recombinant Human ??-Antitrypsin (AAT) from Escherichia coli -- Quantification of Total Human Alpha-1 Antitrypsin by Sandwich ELISA -- Quantification of Murine AAT by Direct ELISA -- Quantification of Z-AAT by a Z-Specific ?쏶andwich??ELISA -- Semi-Quantitation of Monomer and Polymer Alpha-1 Antitrypsin by Centrifugal Separation and Assay by Western Blot of Soluble and Insoluble Components -- Electrophoresis- and FRET-Based Measures of Serpin Olymerization -- Therapeutics: Alpha-1 Antitrypsin Augmentation Therapy -- Therapeutic Option in Alpha-1 Antitrypsin Liver Transplantation -- Therapeutics: Gene Therapy for Alpha-1 Antitrypsin Deficiency.
  • 일반주제명
    Medicine.
    Molecular biology.
    Biomedicine.
    Molecular Medicine.
  • 기본자료 저록
    Springer eBooks
  • 기타형태 저록
    Printed edition: 9781493971619
  • ISBN
    9781493971633
  • 언어
    영어