E-Book

NanoArmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes [electronic resource]

  • Kumar, Challa Vijaya
  • Academic Press
  • 2017
NanoArmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes [electronic resource]
  • 자료유형
    단행본
  • 서명/저자사항
    NanoArmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes[electronic resource] / Challa Vijaya Kumar.
  • 발행사항
    [Cambridge, MA] : Academic Press, 2017.
  • 개인저자
    Kumar, Challa Vijaya
  • 형태사항
    1 online resource.
  • 총서명
    Methods in Enzymology ; 590
  • ISSN
    0076-6879
  • ISBN
    9780128105023
  • 언어
    영어

목차

Chapter One: Encapsulating Proteins in Nanoparticles: Batch by Batch or One by One
Y. Liu and A. Cao

Abstract
1 Introduction
2 “Batch-by-Batch” Encapsulation of Proteins in Silica NPs
3 “One-by-One” Encapsulation of Proteins in NPs
4 Summary and Outlook
Acknowledgments
Chapter Two: Enzyme Adsorption on Nanoparticle Surface Probed by Highly Sensitive Second Harmonic Light Scattering
A. Das, A. Chakrabarti and P.K. Das

Abstract
1 Introduction
2 Techniques for Probing Nanoparticle–Protein Interaction
3 Second Harmonic Light Scattering
4 Sample Preparation
5 Interaction of ADH With GNPs
6 Interaction of Insulin With GNPs
7 Stoichiometry of the Protein–GNP Conjugate
8 Summary and Future Outlook
Acknowledgment
Chapter Three: Armoring Enzymes by Metal–Organic Frameworks by the Coprecipitation Method
M. Hou and J. Ge

Abstract
1 Introduction
2 Equipment
3 Materials
4 Protocol
5 Safety
6 Step1: Preparation of Enzyme@MOF
7 Step2: Preparation of Buffers
8 Step3: Purification of the Product
9 Step4: Characterization of Enzyme@MOF
10 Step5: Measurement of Enzyme Loading in MOFS
11 Step6: Analysis of the Location of Enzymes in MOFS
12 Step7: Determination of Enzyme Activities
13 Conclusions
Chapter Four: Enzyme Armoring by an Organosilica Layer: Synthesis and Characterization of Hybrid Organic/Inorganic Nanobiocatalysts
M.R. Correro, S. Sykora, P.F.-X. Corvini and P. Shahgaldian

Abstract
1 Introduction
2 Equipments and Chemicals
3 Safety Guidelines
4 Procedures
5 Conclusions
Acknowledgments
Chapter Five: Strategies for Biophysical Characterization of Protein–Polymer Conjugates
C. Williams, M.L. Dougherty, K. Makaroff, J. Stapleton, D. Konkolewicz, J.A. Berberich and R.C. Page

Abstract
1 Introduction
2 Biophysical Methods
3 Conclusions
Acknowledgments
Chapter Six: Guide to the Preparation of Molecularly Imprinted Polymer Nanoparticles for Protein Recognition by Solid-Phase Synthesis
J. Xu, P.X. Medina-Rangel, K. Haupt and B.Tse Sum Bui

Abstract
1 Introduction
2 Protocols
3 Physicochemical Characterization of MIP-NPs
4 Evaluation of the Binding Characteristics of MIP-NPs Using a Quartz Crystal Microbalance
5 MIP-NPs Protect Enzyme From Thermal and pH Denaturation
6 Concluding Remarks
7 Notes
Acknowledgments
Chapter Seven: Armored Urease: Enzyme-Bioconjugated Poly(acrylamide) Hydrogel as a Storage and Sensing Platform
K.R. Kunduru, S.N.R. Kutcherlapati, D. Arunbabu and T. Jana

Abstract
1 Introduction
2 Reversible Immobilization
3 Jack Bean Urease: Active Site
4 Immobilization of Jack Bean Urease
5 Methodology
6 Results and Discussion
7 Conclusions and Future Perspectives
Acknowledgments
Chapter Eight: Armored Enzyme–Nanohybrids and Their Catalytic Function Under Challenging Conditions
O.V. Zore, R.M Kasi and C.V. Kumar

Abstract
1 Introduction
2 Methods: Synthesis of Bienzyme–Polymer Conjugates
3 Methods: Synthesis of Bienzyme–Polymer–Graphene Oxide (GOx–HRP–PAA/GO) Hybrid Materials
4 Characterization
5 Conclusions
Acknowledgments
Chapter Nine: Approaches for Conjugating Tailor-Made Polymers to Proteins
M. Paeth, J. Stapleton, M.L. Dougherty, H. Fischesser, J. Shepherd, M. McCauley, R. Falatach, R.C. Page, J.A. Berberich and D. Konkolewicz

Abstract
1 Introduction
2 Polymer Synthetic Procedures
3 Protein Conjugation Methods—Amine Based
4 Polymer Conjugation by GT Using Click Approaches
5 Conjugate Characterization Methods
6 Conclusions
Chapter Ten: NanoArmoring of Enzymes by Polymer-Functionalized Iron Oxide Nanoparticles
G. Premaratne, L. Coats and S. Krishnan

Abstract
1 Introduction
2 NanoArmoring by Making Polymer–Enzyme Conjugates
3 MNP–Polymer–Enzyme Conjugates
4 Covalent vs Noncovalent Immobilization of Peroxidase Active Proteins on PolyMNPs. What Difference Does It Make in the Electrocatalytic Activity and Kinetics?
5 Effect of MNP Size on Activity and Recovery of GOx (Park, McConnell, Boddohi, Kipper, & Johnson, 2011)
6 Summary
7 Future Outlook
Acknowledgments
Chapter Eleven: Expression of Cellulolytic Enzyme as a Fusion Protein That Reacts Specifically With a Polymeric Scaffold
P. Katyal, Y. Yang, O. Vinogradova and Y. Lin

Abstract
1 Introduction
2 Materials
3 Methods
4 Future Outlook
Acknowledgments
Chapter Twelve: Nanoarmoring of Proteins by Conjugation to Block Copolymer Micelles
N. Suthiwangcharoen and R. Nagarajan

Abstract
1 Introduction
2 Synthesis of Block Copolymer–Protein (BSA) Conjugate
3 Characterization of Block Copolymer–Protein Conjugate
4 Characterization of Protein–Polymer Conjugate Micelle
5 Conclusions
Acknowledgments
Chapter Thirteen: Semisynthetic Enzymes by Protein–Peptide Site-Directed Covalent Conjugation: Methods and Applications
J.M Palomo

Abstract
1 Introduction
2 Protein–Peptide Site-Directed Covalent Conjugation
3 The New Semisynthetic Lipases in Biotransformations
4 Conclusions
Acknowledgments
Chapter Fourteen: Transgultaminase-Mediated Nanoarmoring of Enzymes by PEGylation
A. Grigoletto, A. Mero, K. Maso and G. Pasut

Abstract
1 Introduction
2 Materials and Reagents
3 Methods
Chapter Fifteen: Polymer-Based Protein Engineering: Synthesis and Characterization of Armored, High Graft Density Polymer–Protein Conjugates
S. Carmali, H. Murata, C. Cummings, K. Matyjaszewski and A.J. Russell

Abstract
1 Introduction
2 Protocols
3 Protein–Polymer-Based Engineering
4 Conclusions
Acknowledgment
Chapter Sixteen: Nano-Armoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes
K. Raghupathi and S. Thayumanavan

Abstract
1 Introduction
2 Covalent Conjugation
3 Electrostatic Complexation
4 Noncovalent Entrapment
5 Conclusions
Acknowledgments
Chapter Seventeen: Nanoarmored Enzymes for Organic Enzymology: Synthesis and Characterization of Poly(2-Alkyloxazoline)–Enzyme Conjugates
M. Leurs and J.C. Tiller

Abstract
1 Introduction
2 Synthesis of Poly(2-Alkyloxazoline)s With an NH2 End Group
3 Conjugation of Enzymes With Poly(2-Alkyloxazoline)s
4 Conjugate Characterization
5 Activity Evaluation of Poly(2-Alkyloxazoline)–Enzyme Conjugates
6 Conclusions
Acknowledgments
Chapter Eighteen: Preparation and Applications of Dendronized Polymer–Enzyme Conjugates
A. Küchler, D. Messmer, A.D. Schlüter and P. Walde

Abstract
1 Introduction
2 The Denpol de-PG2
3 The Denpol–Enzyme Conjugate de-PG2-BAH-proK
4 Synthesis and Characterization of Denpols Carrying Other Types of Enzymes
5 Immobilization of de-PG2-BAH-proK on Silicate Surfaces
6 Entrapment of Denpol–Enzyme Conjugates Inside Phospholipid Vesicles
7 Conclusions and Outlook
Acknowledgments
Chapter Nineteen: Nanoarmoring of Enzymes by Interlocking in Cellulose Fibers With Poly(Acrylic Acid)
C.M. Riccardi, R.M. Kasi and C.V. Kumar

Abstract
1 Introduction
2 Equipment and Reagents
3 Methods
4 Conclusions
Acknowledgments