Plant and food carotenoids: methods and protocols [electronic resource]

  • Rodríguez-Concepción, Manuel , Welsch, Ralf
  • Humana Press
  • 2020
Plant and food carotenoids: methods and protocols [electronic resource]


This volume provides a comprehensive compilation of techniques and protocols used in plant and food carotenoid research. Chapters guide readers through seven major areas on core enzyme activities, analysis of carotenoid profiles, new imaging tools, synthesis and degradation dynamics, biotechnology, nutrition, and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Part I: Introduction
1. Pathways for Carotenoid Biosynthesis, Degradation, and Storage / Tianhu Sun, Yaakov Tadmor, and Li Li

Part II: Activities
2. A Simple in vitro Assay to Measure the Activity of Geranylgeranyl Diphosphate Synthase and Other Short-chain Prenyltransferases / M. Victoria Barja and Manuel Rodriguez-Concepcion
3. Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase in vitro / Maurizio Camagna and Ralf Welsch
4. Improved Expression and Purification of the Carotenoid Biosynthetic Enzyme Z-ISO / Eleanore T. Wurtzel and Jesús Beltrán
5. Determination of In Vitro and In Vivo Activities of Plant Carotenoid Cleavage Oxygenases / Lourdes Gómez-Gómez, Gianfranco Diretto, Oussama Ahrazem, Salim Al-Babili
6. Expression and Characterization of Mammalian Carotenoid Cleavage Dioxygenases / Linda Thomas, Srinivasagan Ramkumar, and Johannes von Lintig
7. Transportomics for the Characterization of Plant Apocarotenoid Transmembrane Transporters / Olivia Costantina Demurtas, Rita de Brito Francisco, Enrico Martinoia, and Giovanni Giuliano

Part III: Analytics
8. Applications of Visible Spectroscopy and Colour Measurements in the Assessments of Carotenoid Levels in Foods / Paula Mapelli-Brahm, Francisco J. Rodríguez-Pulido, Carla M. Stinco; Francisco J. Heredia, and Antonio J. Meléndez-Martínez
9. A Routine Method for the Extraction and HPLC-DAD Profiling of Major Plant and Food Carotenoids / Dámaso Hornero-Méndez
10. Acquisition of Mass Spectrometry Data of Carotenoids: A Focus on Big Data Management / Antonio Pérez-Gálvez, Isabel Viera, and María Roca
11. cis/trans Carotenoid Extraction, Purification, Detection, Quantification, and Profiling in Plant Tissues / Yagiz Alagoz, Namraj Dhami, Chris Mitchell, and Christopher I Cazzonelli
12. Determination of Plant Volatile Apocarotenoids / José L. Rambla and Antonio Granell
13. A Method for Extraction and LC-MS Based Identification of Carotenoid-Derived Dialdehydes in Plants / Jianing Mi, Kun-Peng Jia, Aparna Balakrishna, and Salim Al-Babili
14. Fractionation of Tomato Fruit Chromoplasts / Karel De Pourcq and Albert Boronat
15. Quantification of Strigolactones / Carlos Rial, Rosa M. Varela, José M. G. Molinillo, Alexandra G. Durán and Francisco A. Macías
16. Carotenoid and Apocarotenoid Analysis by SFE-SFC-QqQ/MS / Daniele Giuffrida, Mariosimone Zoccali, and Luigi Mondello

Part IV: Imaging
17. Elucidating Carotenoid Biosynthetic Enzyme Localization and Interactions Using Fluorescent Microscopy / Maria Shumskaya, Rena F. Quinlan, and Eleanore T. Wurtzel
18. Visualization of Carotenoid-storage Structures in Fruits by Transmission Electron Microscopy / Joanna Lado, Jaime Zacarias, María Jesús Rodrigo, and Lorenzo Zacarias
19. Light Microscopy and Raman Imaging of Carotenoids in Plant Cells in situ and in Released Carotene Crystals / Tomasz Oleszkiewicz, Marta Z. Pacia, Ewa Grzebelus, and Rafal Baranski

Part V: Dynamics
20. Carotenoid Isotopologue Profiling in 13C-labeled Leaf Extracts by LC-MS and LC-FTICR-MS / Björn Thiele and Shizue Matsubara
21. Quantification of Carotenoid Pathway Flux in Green and Non-green Systems / Julian Koschmieder and Ralf Welsch
22. Histochemical and Molecular Quantification of Arbuscular Mycorrhiza Symbiosis / Juan M. García, María J. Pozo, and Juan A. López-Ráez

Part VI: Biotechnology
23. Use of Potyvirus Vectors to Produce Carotenoids in Plants / José-Antonio Daròs
24. Agrobacterium tumefaciens Mediated Stable Transformation of Daucus carota / Christian Gonzalez-Calquin and Claudia Stange
25. CRISPR-Cas-Mediated Gene Knockout in Tomato / Gwen Swinnen, Thomas Jacobs, Laurens Pauwels, and Alain Goossens
26. Modulation of Activity of a Carotenoid Pathway Through the use of the TET-on Regulatory System: Application in the Fungus Fusarium fujikuroi / Julia Marente, Pedro Ortega, Javier Pardo-Medina, Javier Avalos, and M. Carmen Limón

Part VII: Nutrition and Health
27. Intervention Studies in Humans / Begoña Olmedilla-Alonso
28. In vitro Digestion for Control and Monitoring of Food Effects In Relation To Micellarization Index of Carotenoids / Antonio Pérez-Gálvez and Javier Fontecha
29. Evaluation of Carotenoids Protection against Oxidative Stress in the Animal Model Caenorhabditis elegans / Patricia Martorell, Silvia Llopis, José Vicente Gil, Salvador Genovés, Daniel Ramón, Lorenzo Zacarías, and María Jesús Rodrigo
30. Mouse Models to Study Antiobesogenic Effects of Carotenoids / Joan Ribot, Bojan Stojnic, Andreu Palou, and M. Luisa Bonet