Diabetic nephropathy : methods and protocols [electronic resource]

  • Gnudi, Luigi , Long, David A.
  • Humana Press
  • 2020
Diabetic nephropathy : methods and protocols [electronic resource]


This book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches. The collection also explores powerful techniques to image the kidney, such as traditional histological techniques as well as electron, confocal, and two-photon microscopy, pathophysiology of the diabetic kidney, and gene editing and regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Diabetic Nephropathy: An Overview / Manpreet K. Sagoo, Luigi Gnudi

Experimental Models to Study Diabetic Nephropathy
Modeling Podocyte Biology Using Drosophila Nephrocytes / Paul S. Hartley, Richard J. Coward
A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros / Maria Kolatsi-Joannou, Daniel Osborn
Modeling Human Diabetic Kidney Disease by Combining Hyperglycemia and Hypertension in a Transgenic Rodent Model / Carolynn Cairns, Bryan Conway
Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy / Elisavet Vasilopoulou

Techniques to Image the Diabetic Kidney
Histological Examination of the Diabetic Kidney / Camillo Carrara, Mauro Abbate, Sara Conti, Daniela Rottoli, Paola Rizzo, Gianfranco Marchetti
Sample Preparation and Stereological Methods for the Study of Glomerular Ultrastructure Using Electron Microscopy / Kathryn E. White
Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopy / Daniyal J. Jafree, David A. Long, Peter J. Scambler, Dale Moulding

Assessing the Function and Pathophysiological Properties of the Diabetic Kidney
Transcutaneous Measurement of Glomerular Filtration Rate in Rodents / Cristina Daniele, Daniela Nardozi, Angelo Torelli, Arif ul Maula Khan, Norbert Gretz
An In Vitro Method to Analyze Glucose Uptake in Podocytes / Abigail C. Lay, Richard J. Coward
Glomerular Endothelial Cells: Assessment of Barrier Properties In Vitro / Raina D. Ramnath, Simon C. Satchell
Methods to Detect Endoplasmic Reticulum Stress and Apoptosis in Diabetic Nephropathy / Khurrum Shahzad, Sanchita Ghosh, Akash Mathew, Berend Isermann
Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease / Karina Barreiro, Tobias B. Huber, Harry Holthofer
Examining Cell-Cell Interactions in the Kidney Using AFM Single-Cell Force Spectroscopy / Eleftherios Siamantouras, Claire E. Hills, Kuo-Kang Liu, Paul E. Squires

Transcriptome, Metabolic, and Proteomic Profiling of the Diabetic Kidney
Genetic Strategies to Understand Human Diabetic Nephropathy: Wet-Lab Approaches / Laura J. Smyth, Katie Kerr, Seamus Duffy, Jill Kilner, Amy Jayne McKnight
Genetic Strategies to Understand Human Diabetic Nephropathy: In Silico Strategies for Molecular Data—Association Studies / Marisa Canadas-Garre, Laura J. Smyth, Kerry Anderson, Katie Kerr, Amy Jayne McKnight
Assessment of Urinary MicroRNAs by Quantitative Polymerase Chain Reaction in Diabetic Nephropathy Patients / Lucy Jade Newbury, Alexa Wonnacott, Kate Simpson, Timothy Bowen, Donald Fraser
Metabolomic and Proteomic Techniques for Establishing Biomarkers and Improving Our Understanding of Pathophysiology in Diabetic Nephropathy / Justyna Siwy, Linda Ahonen, Pedro Magalhães, Maria Frantzi, Peter Rossing

New Approaches: Gene Editing and Stem Cells to Study Diabetic Kidney Disease
Formation of Mature Nephrons by Implantation of Human Pluripotent Stem Cell-Derived Progenitors into Mice / Ioannis Bantounas, Edina Silajdžić, Adrian S. Woolf, Susan J. Kimber
Generating Mutant Renal Cell Lines Using CRISPR Technologies / Nuria Perretta-Tejedor, Grace Freke, Marian Seda, David A. Long, Dagan Jenkins