Chimeric antigen receptor T cells : methods and protocols [electronic resource]

  • Swiech, Kamilla , Malmegrim, Kelen Cristina Ribeiro , Picanço-Castro, Virgínia
  • Humana Press
  • 2020
Chimeric antigen receptor T cells : methods and protocols [electronic resource]


This volume provides comprehensive methods from expert scientists working in the Chimeric Antigen Receptor T Cell (CAR-T Cell) field. Chapters guide readers through the state-of-art of CAR-T cell technology, CAR design and vector production, CAR-T cell generation and manufacturing, CAR-T cell characterization, and quality control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


CAR-T Cells for Cancer Treatment: Current Design and Next Frontiers
Selection of Antibody Fragments for CAR-T Cell Therapy from Phage Display Libraries
Phase I/II Manufacture of Lentiviral Vectors Under GMP in an Academic Setting
Production of Lentivirus for the Establishment of CAR-T Cells
Optimized Production of Lentiviral Vectors for CAR-T Cell
Lentiviral Vector Production in Suspension Culture Using Serum-Free Medium for the Transduction of CAR-T Cells
In Vitro-Transcribed (IVT)-mRNA CAR Therapy Development
Generation of Chimeric Antigen Receptor T Cells Using Gammaretroviral Vectors
Generation of CAR+ T Lymphocytes Using the Sleeping Beauty Transposon System
Platforms for Clinical-Grade CAR-T Cell Expansion
CAR-T Cell Expansion in a Xuri Cell Expansion System W25
Methods and Process Optimization for Large-Scale CAR T Expansion Using the G-Rex Cell Culture Platform
Replication-Competent Lentivirus Analysis of Vector-Transduced T Cell Products Used in Cancer Immunotherapy Clinical Trials
Immunophenotypic Analysis of CAR-T Cells
Approaches of T Cell Activation and Differentiation for CAR-T Cell Therapies
Determination of Cytotoxic Potential of CAR-T Cells in Co-cultivation Assays
Analysis of CAR-Mediated Tonic Signaling
Generation of Tumor Cells Expressing Firefly Luciferase (fLuc) to Evaluate the Effectiveness of CAR in a Murine Model
Analysis of Antitumor Effects of CAR-T Cells in Mice with Solid Tumors