Cell reprogramming for immunotherapy : methods and protocols [electronic resource]

  • Katz, Samuel G. , Rabinovich, Peter M.
  • Humana Press
  • 2020
Cell reprogramming for immunotherapy : methods and protocols [electronic resource]


This volume details key protocols for developing strategies in immunotherapy. Chapters guide the readers through protocols related to various DNA, RNA and protein methods to reprogram the immune system, immune cells, analyzing the effect of the reprogrammed cells, and key methods to consider and analyze patients enrolled in clinical trials with novel immunotherapy regimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Reprogramming T and NK Cells
T Cell Reprogramming Against Cancer / Samuel G. Katz, Peter M. Rabinovich
Identification of Cell Surface Targets for CAR T Cell Immunotherapy / Diana C. DeLucia, John K. Lee
Microfluidic Approach for Highly Efficient Viral Transduction / Reginald Tran, Wilbur A. Lam
Single-Cell Cytokine Analysis to Characterize CAR-T Cell Activation / Amanda Finck, Rong Fan
Testing the Specificity of Compounds Designed to Inhibit CPT1A in T Cells / Roddy S. O’Connor, Michael C. Milone
Engineering of Natural Killer Cells for Clinical Application / Noriko Shimasaki, Dario Campana
Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells / Arash Nanbakhsh, Subramaniam Malarkannan
In Vivo Assessment of NK Cell-Mediated Cytotoxicity by Adoptively Transferred Splenocyte Rejection / Nathan J. Schloemer, Alex M. Abel, Monica S. Thakar, Subramaniam Malarkannan
Immunomodulation of NK Cell Activity / Carolina I. Domaica, Jessica M. Sierra, Norberto W. Zwirner, Mercedes B. Fuertes

Reprogramming Diverse Immunocytes
An Overview of Advances in Cell-Based Cancer Immunotherapies Based on the Multiple Immune-Cancer Cell Interactions / Jialing Zhang, Stephan S. Späth, Sherman M. Weissman, Samuel G. Katz
Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy / Douglas Hanlon, Olga Sobolev, Patrick Han, Alessandra Ventura, Aaron Vassall, Nour Kibbi et al.
Reprogramming Exosomes for Immunotherapy / Qinqin Cheng, Xiaojing Shi, Yong Zhang
Nanoparticles for Immune Cell Reprogramming and Reengineering of Tumor Microenvironment / Ketki Bhise, Samaresh Sau, Rami Alzhrani, Arun K. Iyer
CRISPR/Cas9 Gene Targeting in Primary Mouse Bone Marrow-Derived Macrophages / Will Bailis
Genome-Wide CRISPRi/a Screening in an In Vitro Coculture Assay of Human Immune Cells with Tumor Cells / Jialing Zhang, Stephan S. Späth, Samuel G. Katz
The Propagation and Quantification of Two Emerging Oncolytic Viruses: Vesicular Stomatitis (VSV) and Zika (ZIKV) / Robert E. Means, Sounak Ghosh Roy, Samuel G. Katz

Monitoring Reprogrammed Immune Cells In Vivo
Radiolabeling and Imaging of Adoptively Transferred Immune Cells by Positron Emission Tomography / Amer M. Najjar
Functional Analysis of Human Hematopoietic Stem Cells In Vivo in Humanized Mice / Yuanbin Song, Rana Gbyli, Xiaoying Fu, Stephanie Halene

Clinical Design for Cell Therapy
Monitoring Allogeneic CAR-T Cells Using Flow Cytometry / Agnieszka Jozwik, Alan Dunlop, Katy Sanchez, Reuben Benjamin
Key Factors in Clinical Protocols for Adoptive Cell Therapy in Melanoma / Bryden Considine, Michael E. Hurwitz
Place of Academic GMP Facilities in Modern Cell Therapy / Alexey Bersenev, Andrew Fesnak