E-Book

MERS Coronavirus Methods and Protocols [electronic resource] :

  • Vijay, Rahul. editor.
  • Humana
  • 2020
MERS Coronavirus Methods and Protocols [electronic resource] :
  • 자료유형
    단행본
  • 서명/저자사항
    MERS Coronavirus[electronic resource] :Methods and Protocols / edited by Rahul Vijay.
  • 판차
    1st ed. 2020.
  • 발행사항
    New York, NY : Humana, 2020.
  • 개인저자
    Vijay, Rahul., editor., edt, http://id.loc.gov/vocabulary/relators/edt
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    XI, 224 p. 40 illus., 27 illus. in color. .
  • 총서명
    Methods in Molecular Biology,1064-3745 ; 2099
  • 내용주기
    Studying Evolutionary Adaptation of MERS-CoV -- Evaluating MERS-CoV Entry Pathways -- Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing -- Crystallization and Structural Determination of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein -- Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV -- Deducing the Crystal Structure of MERS-CoV Helicase -- Antigen Capture Enzyme-linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans -- Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR -- Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay -- Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera -- Qualitative and Quantitative Determination of MERS-CoV S1-specific Antibodies using ELISA -- Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced Acute Respiratory Distress Syndrome -- Development of a Mouse Adapted MERS Coronavirus -- Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection -- Evaluation of Activation and Inflammatory Activity of Myeloid Cells DuringPathogenic Human Coronavirus Infection -- Histopathologic Evaluation and Scoring of Viral Lung Infection. .
  • 일반주제명
    Immunology.
    Virology.
    Immunology. http://scigraph.springernature.com/things/product-market-codes/B14000
    Virology. http://scigraph.springernature.com/things/product-market-codes/B22003
  • 기본자료 저록
    Springer eBooks
  • 기타형태 저록
    Printed edition: 9781071602102
  • ISBN
    9781071602119
  • 언어
    영어

초록

This volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quantitation of virus and anti-viral factors, and mouse models for MERS -coronavirus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MERS Coronavirus: Methods and Protocols aims to ensure successful results in the further study of this vital field.