Lectin Purification and Analysis : Methods and Protocols [electronic resource]

  • Hirabayashi, Jun. editor.
  • Springer US : Imprint: Humana
  • 2020
Lectin Purification and Analysis : Methods and Protocols [electronic resource]


This volume presents a series of experimental protocols for glycan analysis using lectins and the functional analysis of lectins. A key feature in this book are two comprehensive overview chapters on lectin structures and a list of the all the lectins—these chapters discuss the latest findings in the field. The remainder of the book covers topics such as lectins from mammals, non-mammalian animals; plants, algae, fungi, protists, bacteria, and viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Lectin Purification and Analysis: Methods and Protocols is a valuable tool for any scientist interested in learning more about these life systems.


Structural Database for Lectins and the UniLectin Web Platform / François Bonnardel, Serge Perez, Frédérique Lisacek, Anne Imberty
Purification of Sugar-Binding Peptides from L-Type Lectins / Kazuo Yamamoto
Recombinant Expression and Purification of Animal Intracellular L-Type Lectins / Tadashi Satoh, Koichi Kato
Frontal Affinity Chromatography: A Highly Suitable Retardation Phenomenon-Based Research Tool for Analyzing Weak Interactions Between Biomolecules / Kenichi Kasai
Metazoan Soluble β-Galactoside-Binding Lectins, Galectins: Methods for Purification, Characterization of Their Carbohydrate-Binding Specificity, and Probing Their Ligands / Guillaume St-Pierre, Ann Rancourt, Sachiko Sato
Expression, S-Nitrosylation, and Measurement of S-Nitrosylation Ratio of Recombinant Galectin-2 / Mayumi Tamura, Yoichiro Arata
Expression and Purification of Full-Length and Domain-Fragment Recombinant Pentraxin 3 (PTX3) Proteins from Mammalian and Bacterial Cells / Kenji Daigo, Takao Hamakubo
Identification of Siglec Cis-Ligands by Proximity Labeling / Amin Alborzian Deh Sheikh, Chizuru Akatsu, Takeshi Tsubata
Preparation of Recombinant Siglecs and Identification of Their Ligands / Lan-Yi Chang, Penk Yeir Low, Deepa Sridharan, Kaia Gerlovin, Takashi Angata
Purification, Quantification, and Functional Analysis of Collectins / Katsuki Ohtani, Nobutaka Wakamiya
Selectin-Binding Assay by Flow Cytometry / Keiichiro Sakuma, Reiji Kannagi
Direct Binding Analysis Between C-Type Lectins and Glycans Using Immunoglobulin Receptor Fusion Proteins / Miyuki Watanabe, Zakaria Omahdi, Sho Yamasaki
Expression and Characterization of Hyaluronan-Binding Protein Involved in Hyaluronan Depolymerization: HYBID, Alias KIAA1199 and CEMIP / Susana de Vega, Hiroyuki Yoshida, Yasunori Okada
Paracoccin: Purification and Validation of Its Lectin and Enzymatic Properties / Nayla de Souza Pitangui, Fabrício Freitas Fernandes, Relber Aguiar Gonçales, Maria Cristina Roque-Barreira
In Vitro Mannosidase Assay of EDEMs: ER Degradation-Enhancing α-Mannosidase-Like Proteins / Nobuko Hosokawa
Galactose-Specific, Hemolytic Lectin CEL-III from Cucumaria echinata / Tomomitsu Hatakeyama
Simple and Rapid Detection of Glycoforms by “Lectin Inhibition” Assay / Kyoka Hoshi, Yasuhiro Hashimoto, Hiromi Ito
Glycoform-Specific Visualization in Immunohistochemistry by “Lectin Inhibition” / Hiromi Ito, Kyoka Hoshi, Yasuhiro Hashimoto, Takashi Honda
Botulinum Hemagglutinin: Critical Protein for Adhesion and Absorption of Neurotoxin Complex in Host Intestine / Sho Amatsu, Yukako Fujinaga
Functional Analysis of Botulinum Hemagglutinin (HA) / Takuhiro Matsumura, Yukako Fujinaga
Purification and Functional Characterization of the Effects on Cell Signaling of Mytilectin: A Novel β-Trefoil Lectin from Marine Mussels / Yuki Fujii, S. M. Abe Kawsar, Imtiaj Hasan, Hideaki Fujita, Marco Gerdol, Yasuhiro Ozeki
Lectin-Type Ubiquitin Ligase Subunits: Fbs Proteins and Their Applications for Use / Yukiko Yoshida
F-Type Lectins: Structure, Function, and Evolution / Gerardo R. Vasta, Chiguang Feng
Purification and Biochemical Characterization of Selected F-Type Lectins / Chiguang Feng, Gerardo R. Vasta
LecA (PA-IL): A Galactose-Binding Lectin from Pseudomonas aeruginosa / Sakonwan Kuhaudomlarp, Emilie Gillon, Annabelle Varrot, Anne Imberty
Glycan Recognition and Application of P-Type Lectins / Kei Kiriyama, Kohji Itoh
Purification and Assays of Tachylectin-5 / Shun-ichiro Kawabata, Toshio Shibata
Preparation of Soluble Malectin and Its Tetramer / Sheng-Ying Qin, Dan Hu, Kazuo Yamamoto
Calnexin/Calreticulin and Assays Related to N-Glycoprotein Folding In Vitro / Yoshito Ihara, Midori Ikezaki, Maki Takatani, Yukishige Ito
Purification and Assays of Tachylectin-2 / Shun-ichiro Kawabata, Toshio Shibata
Purification and Assays of Tachycitin / Shun-ichiro Kawabata, Toshio Shibata
Methods for Purifying Datura stramonium Agglutinin and Producing Recombinant Agglutinin Protein in a Heterologous Plant Host / Suguru Oguri
ZG16p, an Animal Homologue of Plant β-Prism Fold Lectins: Purification Methods of Natural and Recombinant ZG16p and Inhibition Assay of Cancer Cell Growth Using ZG16p / Akiko Mito, Kaori Kumazawa-Inoue, Kyoko Kojima-Aikawa
ArtinM: Purification and Evaluation of Biological Activities / Thiago Aparecido da Silva, Patrícia Kellen Martins Oliveira-Brito, Sandra Maria de Oliveira Thomaz, Maria Cristina Roque-Barreira
Bacterial Expression of Rhamnose-Binding Lectin from Catfish Eggs / Shigeki Sugawara, Takeo Tatsuta, Masahiro Hosono
A Bioassay for Determining Symbiotic Zooxanthellae Shape Control Using Lectin SLL-2 from the Octocoral Sinularia lochmodes / Mitsuru Jimbo, Ryota Takeuchi, Mayu Yoshino
MIC4 from Toxoplasma gondii: A Lectin Acting as a Toll-Like Receptor Agonist / Flávia Costa Mendonça-Natividade, Rafael Ricci-Azevedo, Maria Cristina Roque-Barreira
Production and Characterization of MIC1: A Lectin from Toxoplasma gondii / Flávia Costa Mendonça-Natividade, Rafael Ricci-Azevedo, Sandra Maria de Oliveira Thomaz, Maria Cristina Roque-Barreira
Affinity Labeling and Purification of Plant Chitin-Binding LysM Receptor with Chitin Octasaccharide Derivatives / Tomonori Shinya, Naoto Shibuya, Hanae Kaku
Purification of GNA-Related Lectins from Natural Sources / Els J. M. Van Damme
Expression, Purification, and Applications of the Recombinant Lectin PVL from Psathyrella velutina Specific for Terminal N-Acetyl-Glucosamine / Oriane Machon, Annabelle Varrot
Yeast Flocculin: Methods for Quantitative Analysis of Flocculation in Yeast Cells / Hiromi Maekawa, Kaoru Takegawa
Pleurotus cornucopiae Mycelial Lectin (PCL-M): Purification and Detection of the Activity on Mycelial Surface / Suguru Oguri
Expression and Purification of a Human Pluripotent Stem Cell-Specific Lectin Probe, rBC2LCN / Hiroaki Tateno
Preparation of Fluorescent Recombinant Shiga Toxin B Subunit and Its Application to Flow Cytometry / Toshiyuki Yamaji
LecB, a High Affinity Soluble Fucose-Binding Lectin from Pseudomonas aeruginosa / Emilie Gillon, Annabelle Varrot, Anne Imberty
Sialoglycovirology of Lectins: Sialyl Glycan Binding of Enveloped and Non-enveloped Viruses / Nongluk Sriwilaijaroen, Yasuo Suzuki
Hemagglutinin Inhibitors are Potential Future Anti-Influenza Drugs for Mono- and Combination Therapies / Nongluk Sriwilaijaroen, Yasuo Suzuki
Preparation and Detection of Glycan-Binding Activity of Influenza Virus / Shin-ichi Nakakita, Nongluk Sriwilaijaroen, Yasuo Suzuki, Jun Hirabayashi
Screening for Components/Compounds with Anti-Rotavirus Activity: Detection of Interaction Between Viral Spike Proteins and Glycans / Keita Yamada, Junko Nio-Kobayashi, Mizuho Inagaki
ELISA-Based Methods to Detect and Quantify Norovirus Virus-Like Particle Attachment to Histo-Blood Group Antigens / Haruko Shirato
FAM3B/PANDER-Like Carbohydrate-Binding Domain in a Glycosyltransferase, POMGNT1 / Hiroshi Manya, Naoyuki Kuwabara, Ryuichi Kato, Tamao Endo
Mannose-Specific Oyster Lectin CGL1 / Hideaki Unno, Tomomitsu Hatakeyama
Receptor-Binding Assays of Enterovirus D68 / Tadatsugu Imamura, Michiko Okamoto, Hitoshi Oshitani
Large-Scale Expression and Purification of Mumps Virus Hemagglutinin-Neuraminidase for Structural Analyses and Glycan-Binding Assays / Marie Kubota, Takao Hashiguchi
Purification of AJLec: A Novel Galactose-Specific Lectin from the Sea Anemone Anthopleura japonica / Hideaki Unno
Annexin Lectins: Ca2+-Dependent Heparin-Binding Activity, Phosphatidylserine-Binding Activity, and Anticoagulant Activity / Moeka Nakayama, Miyuki Tsunooka-Ohta, Kyoko Kojima-Aikawa
Expression, Purification, and Functional Characterization of Tectonin 2 from Laccaria bicolor: A Six-Bladed Beta-Propeller Lectin Specific for O-Methylated Glycans / Therese Wohlschlager, Alexander Titz, Markus Künzler, Annabelle Varrot
The OAAH Family: Anti-Influenza Virus Lectins / Yuichiro Sato, Makoto Hirayama, Kinjiro Morimoto, Kanji Hori