Aging: Methods and Protocols [electronic resource]

  • Curran, Sean P. editor.
  • Springer US : Imprint: Humana
  • 2020
Aging: Methods and Protocols [electronic resource]


This volume details the impact of aging on biological system and to understand the basis of this diversity with the goal of elucidating mechanisms to optimize health. Chapters guide readers through different animal models and methodologies to examine aging at the organism, tissue, cellular, and molecular levels across the lifespan. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Aging: Methods and Protocols aims to ensure successful results in the further study of this vital field.


Measuring the Replicative Lifespan of Saccharomyces cerevisiae Using the HYAA Microfluidic Platform / Ruofan Yu, Myeong Chan Jo, Weiwei Dang
Analysis of Lifespan in C. elegans: Low- and High-Throughput Approaches / Adam B. Cornwell, Andrew V. Samuelson
A Simple Apparatus for Individual C. elegans Culture / William E. Pittman, Drew B. Sinha, Holly E. Kinser, Nisha S. Patil, Eric S. Terry, Isaac B. Plutzer et al.
Analysis of Drosophila melanogaster Lifespan / Gary N. Landis, Devon Doherty, John Tower
Mouse Fitness as Determined Through Treadmill Running and Walking / Joseph C. Reynolds, Changhan Lee
Human Population Genetics in Aging Studies for Molecular Biologists / Brendan Miller, Amin Haghani, Jennifer Ailshire, T. Em Arpawong
Design and Analysis of Pharmacological Studies in Aging / Khalyd J. Clay, Michael Petrascheck
Methods for Assessing Fertility in C. elegans from a Single Population / Chia-An Yen, Sean P. Curran
Metabolic Assessment of Lipid Abundance and Distribution / James D. Nhan, Sean P. Curran
Quantitative Profiling of Lipid Species in Caenorhabditis elegans with Gas Chromatography–Mass Spectrometry / Elizabeth C. Pino, Alexander A. Soukas
Assessing Insulin and Glucose Tolerance in the Aging Mouse / Roberta Buono, Sebastian Brandhorst
High-Throughput Assessment of Changes in the Caenorhabditis elegans Gut Microbiome / Fan Zhang, Jessica L. Weckhorst, Adrien Assié, Anastasia S. Khodakova, Mario Loeza-Cabrera, Daniela Vidal et al.
Measurements of Innate Immune Function in C. elegans / Kyle J. Foster, Deborah L. McEwan, Read Pukkila-Worley
Measuring Phagocytosis in Bone Marrow-Derived Macrophages and Peritoneal Macrophages with Aging / Ryan Lu, Nirmal K. Sampathkumar, Bérénice A. Benayoun
Chromatin Immunoprecipitation from Caenorhabditis elegans Somatic Cells / Mintie Pu, Siu Sylvia Lee
Transcriptional Profiling of C. elegans Adult Cells and Tissues with Age / Rachel Kaletsky, Coleen T. Murphy
Asessing Tissue-Specific Autophagy Flux in Adult Caenorhabditis elegans / Jessica T. Chang, Malene Hansen, Caroline Kumsta
Assay Development and Measurement of the Aging Biomarker Humanin / Brendan Miller, Junxiang Wan
Staining and Quantification of β-Amyloid Pathology in Transgenic Mouse Models of Alzheimer’s Disease / Amy Christensen, Christian J. Pike
Multimodal Imaging of Cerebral Microhemorrhages and White Matter Degradation in Geriatric Patients with Mild Traumatic Brain Injury / Maria Calvillo, Di Fan, Andrei Irimia
Detection of HNE Modification of Proteins in Aging Mouse Tissues: A Western Blot-Based Approach / Hongqiao Zhang, Natalie Lyn, Amin Haghani, Henry Jay Forman
Measurements of Hydrogen Peroxide and Oxidative DNA Damage in a Cell Model of Premature Aging / Juan Manuel Iglesias-Pedraz, Lucio Comai
Integrating Longitudinal Population Studies of Aging in Biological Research / Amin Haghani, Brendan Miller, T. Em Arpawong, Jennifer Ailshire