E-Book

Arabidopsis Protocols [electronic resource]

  • Sanchez-Serrano, Jose J. editor , Salinas, Julio. editor
  • Humana
  • 2021
Arabidopsis Protocols [electronic resource]
  • 자료유형
    단행본
  • 서명/저자사항
    Arabidopsis Protocols[electronic resource] / edited by Jose J. Sanchez-Serrano, Julio Salinas.
  • 판차
    4th ed. 2021.
  • 발행사항
    New York, NY : Humana, 2021.
  • 개인저자
    Sanchez-Serrano, Jose J., editor, Salinas, Julio., editor
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    1 online resource.
  • 총서명
    Methods in Molecular Biology,1064-3745 ; 2200
  • 내용주기
    Handling Arabidopsis and other Brassicaceae: Growth, Preservation of Seeds, Transformation and Genetic Crosses -- Bioinformatic Tools in Arabidopsis Research -- Analyses of Natural Variation: Field Experiments and Nucleotide Diversity for Your Favorite Gene -- Non-sterile Grafting Methods for Arabidopsis -- CRISPR/Cas9-based Genome Editing Toolbox for Arabidopsis thaliana -- Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration -- Transient Expression of Fluorescent Fusion Proteins in Arabidopsis Protoplasts -- Photosynthetic and Heterotrophic Cell Suspension Cultures -- Genome-wide Association Studies in Arabidopsis thaliana: Statistical Analysis and Network-Based Augmentation of Signals -- Visualising and Measuring Single Locus Dynamics in Arabidopsis thaliana -- Identification and Quantification of Small RNAs -- Flow Cytometry and Sorting in Arabidopsis -- Live Imaging of Arabidopsis Leaf and Vegetative Meristem Development -- Using Genetically Encoded Fluorescent Biosensors for Quantitative in vivo Imaging -- Using Tripartite Split-sfGFP for the Study of Membrane Protein?밣rotein Interactions -- High-pressure Freezing and Freeze Substitution for Transmission Electron Microscopy Imaging and Immunogold-labeling -- Atomic Force Microscopy to Study Cell Wall Mechanics in Plants -- Identification and Characterization of Reproductive Mutations in Arabidopsis -- Metabolomic Analysis of Natural Variation in Arabidopsis -- Untargeted Metabolomics of Arabidopsis Stomatal Immunity -- Biotin-based Proximity Labeling Of Protein Complexes in Planta -- Phosphoproteomic Analysis of Plant Membranes -- RNA-binding Protein Immunoprecipitation and High Throughput Sequencing. .
  • 일반주제명
    Plant science.
    Botany.
    Plant Sciences
  • 기본자료 저록
    Springer Nature eBook
  • ISBN
    9781071608807
  • 언어
    영어

초록

This fourth edition compiles the most recent methodologies developed to exploit the Arabidopsis genome. Chapters detail access to public resources, to genetic, cell biology, biochemical and physiological techniques, and sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Arabidopsis Protocols, Fourth Edition aims to ensure successful results in the further study of this vital field.

목차

Arabidopsis Resources
Handling Arabidopsis and Other Brassicaceae: Growth, Preservation of Seeds, Transformation, and Genetic Crosses / Christopher Calhoun, Deborah Crist, Emma Knee, Julie Miller, Eva Nagy, David E. Somers
Bioinformatic Tools in Arabidopsis Research / G. Alex Mason, Alex Cantó-Pastor, Siobhan M. Brady, Nicholas J. Provart

Genetic Techniques in Arabidopsis
Analyses of Natural Variation: Field Experiments and Nucleotide Diversity for Your Favorite Gene / Carlos Alonso-Blanco, Belén Méndez-Vigo, F. Xavier Picó
Non-sterile Grafting Methods for Arabidopsis / Nien-Chen Huang, Tien-Shin Yu
CRISPR/Cas9-Based Genome Editing Toolbox for Arabidopsis thaliana / Daisuke Miki, Gaurav Zinta, Wenxin Zhang, Fangnan Peng, Zhengyan Feng, Jian-Kang Zhu
Transient Transformation of A. thaliana Seedlings by Vacuum Infiltration / César Bernat-Silvestre, Vanessa De Sousa Vieira, Judit Sánchez-Simarro, Fernando Aniento, María Jesús Marcote
Transient Expression of Fluorescent Fusion Proteins in Arabidopsis Protoplasts / Yonglun Zeng, Changyang Ji, Youshun Lin, Liwen Jiang
Arabidopsis Photosynthetic and Heterotrophic Cell Suspension Cultures / Enrico Cortese, Luca Carraretto, Barbara Baldan, Lorella Navazio
Genome-Wide Association Studies in Arabidopsis thaliana: Statistical Analysis and Network-Based Augmentation of Signals / Tak Lee, Insuk Lee

Molecular and Cell Biological Techniques in Arabidopsis
Visualizing and Measuring Single Locus Dynamics in Arabidopsis thaliana / Anis Meschichi, Stefanie Rosa
Identification and Quantification of Small RNAs / Di Sun, Zeyang Ma, Jiaying Zhu, Xiuren Zhang
Flow Cytometry and Sorting in Arabidopsis / David W. Galbraith, Guiling Sun
Live Imaging of Arabidopsis Leaf and Vegetative Meristem Development / Monica Pia Caggiano, Xiulian Yu, Carolyn Ohno, Pia Sappl, Marcus G. Heisler
Using Genetically Encoded Fluorescent Biosensors for Quantitative In Vivo Imaging / Akira Yoshinari, Jacob Moe-Lange, Thomas J. Kleist, Heather N. Cartwright, David A. Quint, David W. Ehrhardt et al.
Using Tripartite Split-sfGFP for the Study of Membrane Protein–Protein Interactions / Tzu-Yin Liu
High-Pressure Freezing and Freeze Substitution for Transmission Electron Microscopy Imaging and Immunogold-Labeling / Marisa S. Otegui
Atomic Force Microscopy to Study Cell Wall Mechanics in Plants / Mateusz Majda
Identification and Characterization of Reproductive Mutations in Arabidopsis / María Flores-Tornero, Stefanie Sprunck, Thomas Dresselhaus

Biochemical and Physiological Techniques in Arabidopsis
Metabolomic Analysis of Natural Variation in Arabidopsis / Si Wu, Saleh Alseekh, Yariv Brotman, Alisdair R. Fernie
Untargeted Metabolomics of Arabidopsis Stomatal Immunity / Lisa David, Jianing Kang, Sixue Chen
Biotin-Based Proximity Labeling of Protein Complexes in Planta / Madiha Khan, Rajagopal Subramaniam, Darrell Desveaux
Phosphoproteomic Analysis of Plant Membranes / Lin Xi, Waltraud X. Schulze, Xu Na Wu
RNA-Binding Protein Immunoprecipitation and High-Throughput Sequencing / Tino Köster, Dorothee Staiger