E-Book

RNA Tagging : Methods and Protocols [electronic resource]

  • Heinlein, Manfred. editor
  • Humana
  • 2020
RNA Tagging : Methods and Protocols [electronic resource]
  • 자료유형
    단행본
  • 서명/저자사항
    RNA Tagging :Methods and Protocols[electronic resource] / edited by Manfred Heinlein.
  • 판차
    1st ed. 2020.
  • 발행사항
    New York, NY : Humana, 2020.
  • 개인저자
    Heinlein, Manfred., editor
  • 형태사항
    1 online resource.
  • 총서명
    Methods in Molecular Biology,1064-3745 ; 2166
  • 일반주제명
    Proteins.
    Biochemistry.
    Protein Science
    Biochemistry, general
  • 기본자료 저록
    Springer Nature eBook
  • ISBN
    9781071607121
  • 언어
    영어

초록

This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.

목차

Tagging and Application of RNA Probes for Sequence-Specific Visualization of RNAs by Fluorescent In Situ Hybridization
Quantitative Fluorescence In Situ Hybridization Detection of Plant mRNAs with Single-Molecule Resolution
Visualization of Endoplasmic Reticulum-Associated mRNA in Mammalian Cells
Simultaneous Detection of mRNA and Protein in S. cerevisiae by Single Molecule FISH and Immunofluorescence
Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More
Visualization of Transiently Expressed mRNA in Plants Using MS2
New Generations of MS2 Variants and MCP Fusions to Detect Single mRNAs in Living Eukaryotic Cells
In Vivo Imaging of Mobile mRNAs in Plants Using MS2
RNA Imaging with RNase-Inactivated Csy4 in Plants and Filamentous Fungi
Utilizing Potato Virus X to Monitor RNA Movement
A Protocol for Non-Biased Identification of RNAs Transferred between Heterologous Mammalian Cell Types Using RNA Tagging, Cell Sorting, and Sequencing
Synthesizing Fluorescently Labeled dsRNAs and sRNAs to Visualize Fungal RNA Uptake
Labeling of dsRNA for Fungal Uptake Detection Analysis
Using RNA Affinity Purification Followed by Mass Spectrometry to Identify RNA-Binding Proteins (RBPs)
Plant Individual Nucleotide Resolution Crosslinking and Immunoprecipitation to Characterize RNA-Protein Complexes
A Single-Molecule RNA Mobility Assay to Identify Proteins that Link RNAs to Molecular Motors
Proximity-CLIP Provides a Snapshot of Protein-Occupied RNA Elements at Subcellular Resolution and Transcriptome-Wide Scale
Double-Stranded RNA Pull-Down to Characterize Viral Replication Complexes in Plants
CRISPR Guide RNA Design Guidelines for Efficient Genome Editing
Live Cell CRISPR Imaging in Plant Cells with a Telomere-Specific Guide RNA
Live-Cell Imaging of Genomic Loci Using CRISPR/Molecular Beacon Hybrid Systems
Using RNA Tags for Multicolor Live Imaging of Chromatin Loci and Transcription in Drosophila Embryos
Integrated Genome-Scale Analysis and Northern Blot Detection of Retrotransposon siRNAs across Plant Species
Transfection of Small Non-Coding RNAs into Arabidopsis thaliana Protoplasts
In Vivo Reporter System for miRNA-Mediated RNA Silencing
TRAP-SEQ of Eukaryotic Translatomes Applied to the Detection of Polysome-Associated Long Non-Coding RNAs
Posttranscriptional Suzuki-Miyaura Cross-Coupling Yields Labeled RNA for Conformational Analysis and Imaging