E-Book

Stem Cells and Tissue Repair : Methods and Protocols [electronic resource]

  • Kioussi, Chrissa. editor
  • Springer US : Imprint: Humana
  • 2020
Stem Cells and Tissue Repair : Methods and Protocols [electronic resource]
  • 자료유형
    단행본
  • 서명/저자사항
    Stem Cells and Tissue Repair :Methods and Protocols[electronic resource] / edited by Chrissa Kioussi.
  • 판차
    2nd ed. 2020.
  • 발행사항
    New York, NY : Springer US : Imprint: Humana, 2020.
  • 개인저자
    Kioussi, Chrissa., editor
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    XI, 214 p. 36 illus., 34 illus. in color. .
  • 총서명
    Methods in Molecular Biology,1064-3745 ; 2155
  • 내용주기
    Culturing and Manipulating Mouse Embryonic Stem Cells -- Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter -- Engraftable Induced Pluripotent Stem Cell-Derived Neural Precursors for Brain Repair -- In Vitro Direct Reprogramming of Mouse and Human Astrocytes to Induced Neurons -- Preparation of Bioscaffolds Delivering Stem Cells for Neural Regeneration -- Improved Isolation of Human Vascular Wall-Resident Mesenchymal Stem Cells -- In Vitro Generation of Vascular Wall-Typical Mesenchymal Stem Cells (VW-MSC) from Murine Induced Pluripotent Stem Cells through VW-MSC Specific Gene Transfer -- Analysis of Tooth Innervation in Microfluidic Co-Culture Devices -- Peripheral Nerve Regeneration in a Novel Rat Model of Dysphagia -- Healing of Full Thickness Murine Skin Wounds Containing Nanofibers using Splints for Efficient Re-Epithelialization and to Avoid Contracture -- Enrichment and Characterization of Human and Murine Pulmonary Mesenchymal Progenitor Cells (MPC) -- Isolation and Culture of Quiescent Skeletal Muscle Satellite Cells -- Isolation, Culture, Cryopreservation and Identification of Bovine, Murine, and Human Spermatogonial Stem Cells -- Long-Term Ex Vivo Expansion of Murine Spermatogonial Stem Cells in a Simple Serum-Free Medium -- Generating Kidney Organoids from Human Pluripotent Stem Cells Using Defined Conditions -- Painting the Pancreas in Three Dimensions: Whole Mount Immunofluorescence Method -- Induction of Osteoblasts by Direct Reprogramming of Mouse Fibroblasts.
  • 일반주제명
    Stem cells.
    Regenerative medicine.
    Tissue engineering.
    Stem Cells
    Regenerative Medicine/Tissue Engineering
  • 기본자료 저록
    Springer eBooks
  • ISBN
    9781071606551
  • 언어
    영어

초록

This volume looks at a collection of stem cell and regenerative techniques used by both novice and expert scientists. Chapters cover topics such as tissue repaired by expansion and reprogramming; induced pluripotent stem cells driven in neuronal or vascular differentiation; using mesenchymal stem cells to derive skeletal muscle, osteoblasts, and spermatogonial cells; and the technique of monitoring the development of sub-organ microenvironments in the developing pancreas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Stem Cells and Tissue Repair: Methods and Protocols, Second Edition is a valuable resource that provides readers with the latest descriptions and references for exploring this vast field in regenerative medicine

목차

Culturing and Manipulating Mouse Embryonic Stem Cells / Chrissa Kioussi
Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter / Kiel T. Butterfield, Patrick S. McGrath, Chann Makara Han, Igor Kogut, Ganna Bilousova
Engraftable Induced Pluripotent Stem Cell-Derived Neural Precursors for Brain Repair / Ourania Zygogianni, Georgia Kouroupi, Era Taoufik, Rebecca Matsas
In Vitro Direct Reprogramming of Mouse and Human Astrocytes to Induced Neurons / Katerina Aravantinou-Fatorou, Dimitra Thomaidou
Preparation of Bioscaffolds Delivering Stem Cells for Neural Regeneration / Li Yao, Ashley DeBrot
Improved Isolation of Human Vascular Wall–Resident Mesenchymal Stem Cells / Diana Klein
In Vitro Generation of Vascular Wall–Typical Mesenchymal Stem Cells (VW-MSC) from Murine Induced Pluripotent Stem Cells Through VW-MSC–Specific Gene Transfer / Jennifer Steens, Hannes Klump, Diana Klein
Analysis of ToothInnervation in Microfluidic Coculture Devices / Pierfrancesco Pagella, Thimios A. Mitsiadis
Peripheral Nerve Regeneration in a Novel Rat Model of Dysphagia / Kiyoshi Sakai, Takeshi Tsuruta, Junna Watanabe, Yukiko Sugimura, Kohei Sakaguchi, Wataru Katagiri et al.
Healing of Full-Thickness Murine Skin Wounds Containing Nanofibers Using Splints for Efficient Reepithelialization and to Avoid Contracture / Nilika Bhattacharya, Arup K. Indra, Gitali Ganguli-Indra
Enrichment and Characterization of Human and Murine Pulmonary Mesenchymal Progenitor Cells (MPC) / Megan Summers, Karen Helm, Susan M. Majka
Isolation and Culture of Quiescent Skeletal Muscle Satellite Cells / Francisco Hernández-Torres, Lara Rodríguez-Outeiriño, Amelia Aránega
Isolation, Culture, Cryopreservation, and Identification of Bovine, Murine, and Human Spermatogonial Stem Cells / Pedro M. Aponte
Long-Term Ex Vivo Expansion of Murine Spermatogonial Stem Cells in a Simple Serum-Free Medium / Hiroshi Kubota, Kazue Kakiuchi
Generating Kidney Organoids from Human Pluripotent Stem Cells Using Defined Conditions / Sara E. Howden, Melissa H. Little
Painting the Pancreas in Three Dimensions: Whole-Mount Immunofluorescence Method / Maricela Maldonado, Jeffrey D. Serrill, Hung-Ping Shih
Induction of Osteoblasts by Direct Reprogramming of Mouse Fibroblasts / Hui Zhu, Joy Y. Wu