E-Book

Axon Degeneration Methods and Protocols [electronic resource] :

  • Babetto, Elisabetta. editor
  • Springer US : Imprint: Humana
  • 2020
Axon Degeneration Methods and Protocols [electronic resource] :
  • 자료유형
    단행본
  • 서명/저자사항
    Axon Degeneration[electronic resource] :Methods and Protocols / edited by Elisabetta Babetto.
  • 판차
    1st ed. 2020.
  • 발행사항
    New York, NY : Springer US : Imprint: Humana, 2020.
  • 개인저자
    Babetto, Elisabetta., editor
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    XI, 340 p. 80 illus., 58 illus. in color. .
  • 총서명
    Methods in Molecular Biology,1064-3745 ; 2143
  • 내용주기
    Axon Degeneration: Which Method to Choose? -- Axon Degeneration Assays in Superior Cervical Ganglion Explant Cultures -- Microinjection of Superior Cervical Ganglion Neurons for Studying Axon Degeneration -- Assessing Axonal Degeneration in Embryonic Dorsal Root Ganglion Neurons In Vitro -- Viral Transduction of DRG Neurons -- Planning and Analysis of Axon Degeneration Screening Experiments -- A Microfluidic Culture Platform to Assess Axon Degeneration -- A Schwann Cell-Neuron Co-Culture System to Study Neuron-Glia Interaction during Axon Degeneration -- Establishing Myelinating Co-Cultures Using Human iPSC-Derived Sensory Neurons to Investigate Axonal Degeneration and Demyelination -- Preparation of Organotypic Hippocampal Slice Cultures for the Study of CNS Disease and Damage -- Organotypic Culture Assay for Neuromuscular Synaptic Degeneration and Function -- Intracellular Recordings of Postsynaptic Voltage Responses at the Drosophila Neuromuscular Junction -- Ex Vivo Studies of Optic Nerve Axon Electrophysiology -- Ex Vivo Analysis of Axonal Degeneration Using Sciatic and Optic Nerve Preparations -- Measuring Bioenergetic Signatures of Peripheral Nerve Segments by Extracellular Flux Analysis -- Detection of Neutrophils in the Sciatic Nerve Following Peripheral Nerve Injury -- Whole-Tissue Immunolabeling and 3D Fluorescence Imaging to Visualize Axon Degeneration in the Intact, Unsectioned Mouse Tissues -- Transmission Electron Microscopy and Morphometry of the CNS White Matter -- In Vivo Calcium Imaging During Axon Degeneration in Zebrafish -- In Vivo Imaging of Anterograde and Retrograde Axonal Transport in Rodent Peripheral Nerves -- In Vivo Visualization of Moving Synaptic Cargo Complexes within Drosophila Larval Segmental Axons -- A Chemotherapy-Induced Peripheral Neuropathy Model in Drosophila melanogaster -- Models of Axon Degeneration in Drosophila Larvae -- In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster.
  • 일반주제명
    Neurobiology.
    Neurosciences.
    Neurobiology
    Neurosciences
  • 기본자료 저록
    Springer eBooks
  • ISBN
    9781071605851
  • 언어
    영어

초록

This book is a collection of classical as well as innovative methods used to investigate axon degeneration with a particular focus on addressing the common challenges encountered while performing these procedures. Particular attention is devoted to the study of axon loss in several model organisms, as each poses unique challenges and provides powerful advantages. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Axon Degeneration: Methods and Protocols is an ideal guide for facilitating the application and further development of these protocols, which will help the scientific community tackle important questions regarding axon degeneration. Chapters 2, 3, and 20 are available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.

목차

Introduction
Axon Degeneration: Which Method to Choose? / Michael P. Coleman

In Vitro Assays
Axon Degeneration Assays in Superior Cervical Ganglion Explant Cultures / Andrea Loreto, Jonathan Gilley
Microinjection of Superior Cervical Ganglion Neurons for Studying Axon Degeneration / Jonathan Gilley, Andrea Loreto
Assessing Axonal Degeneration in Embryonic Dorsal Root Ganglion Neurons In Vitro / Jung Eun Shin, Yongcheol Cho
Viral Transduction of DRG Neurons / Yo Sasaki
Planning and Analysis of Axon Degeneration Screening Experiments / Lyndah Lovell, John Bramley, William Buchser
A Microfluidic Culture Platform to Assess Axon Degeneration / Yu Yong, Christopher Hughes, Christopher Deppmann
A Schwann Cell–Neuron Coculture System to Study Neuron–Glia Interaction During Axon Degeneration / Elisabetta Babetto
Establishing Myelinating Cocultures Using Human iPSC-Derived Sensory Neurons to Investigate Axonal Degeneration and Demyelination / Alex J. Clark

Ex Vivo Assays
Preparation of Organotypic Hippocampal Slice Cultures for the Study of CNS Disease and Damage / Claire S. Durrant
Organotypic Culture Assay for Neuromuscular Synaptic Degeneration and Function / Kosala N. Dissanayake, Robert Chang-Chih Chou, Rosalind Brown, Richard R. Ribchester
Intracellular Recordings of Postsynaptic Voltage Responses at the Drosophila Neuromuscular Junction / Vera Valakh, Johanna G. Flyer-Adams
Ex Vivo Studies of Optic Nerve Axon Electrophysiology / Chinthasagar Bastian, Sylvain Brunet, Selva Baltan
Ex Vivo Analysis of Axonal Degeneration Using Sciatic and Optic Nerve Preparations / Rodrigo López-Leal, Felipe A. Court
Measuring Bioenergetic Signatures of Peripheral Nerve Segments by Extracellular Flux Analysis / Bogdan Beirowski

In Vivo Assays
Detection of Neutrophils in the Sciatic Nerve Following Peripheral Nerve Injury / Jon P. Niemi, Jane A. Lindborg, Richard E. Zigmond
Whole-Tissue Immunolabeling and 3D Fluorescence Imaging to Visualize Axon Degeneration in the Intact, Unsectioned Mouse Tissues / Ying Cao, Jing Yang
Transmission Electron Microscopy and Morphometry of the CNS White Matter / Julia M. Edgar, Rebecca Sherrard Smith, Ian D. Duncan
In Vivo Calcium Imaging During Axon Degeneration in Zebrafish / Mauricio E. Vargas
In Vivo Imaging of Anterograde and Retrograde Axonal Transport in Rodent Peripheral Nerves / James N. Sleigh, Andrew P. Tosolini, Giampietro Schiavo
In Vivo Visualization of Moving Synaptic Cargo Complexes within Drosophila Larval Segmental Axons / Rupkatha Banerjee, Joseph A. White II, Shermali Gunawardena
A Chemotherapy-Induced Peripheral Neuropathy Model in Drosophila melanogaster / Martha R. C. Bhattacharya
Models of Axon Degeneration in Drosophila Larvae / E. J. Brace, Aaron DiAntonio
In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster / Mary A. Logan, Sean D. Speese