E-Book

Leptospira spp.: Methods and Protocols [electronic resource]

  • Koizumi, Nobuo. editor , Picardeau, Mathieu. editor
  • Humana
  • 2020
Leptospira spp.: Methods and Protocols [electronic resource]
  • 자료유형
    단행본
  • 서명/저자사항
    Leptospira spp.:Methods and Protocols[electronic resource] / edited by Nobuo Koizumi, Mathieu Picardeau.
  • 판차
    1st ed. 2020.
  • 발행사항
    New York, NY : Humana, 2020.
  • 개인저자
    Koizumi, Nobuo., editor, Picardeau, Mathieu., editor
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    1 online resource.
  • 총서명
    Methods in Molecular Biology,1064-3745 ; 2134
  • 내용주기
    Isolation and Culture of Leptospira from Clinical and Environmental Samples -- Core Genome Multi-Locus Sequence Typing Analyses of Leptospira spp. using the Bacterial Isolate Genome Sequence Database -- Use of MALDI-ToF Mass Spectrometry for Identification of Leptospira -- Cell Enumeration of Leptospira by Flow Cytometry -- The Single-Step Method of RNA Purification Applied to Leptospira -- Purification of LPS from Leptospira -- Isolation, Purification, and Characterization of Leptophages -- Creating a Library of Random Transposon Mutants in Leptospira -- Transposon Sequencing in Leptospira interrogans -- Specific Gene Silencing in Leptospira biflexa by RNA-Guided Catalytically inactive Cas9 (dCas9) -- Leptospira spp. Toolbox for Chemotaxis Assay -- In Situ Structural Analysis of Leptospira spp. by Electron Cryotomography -- Measurement of the Cell-Body Rotation of Leptospira -- In Vivo Imaging of Bioluminescent Leptospires -- Cell Monolayer Translocation Assay -- Cell Adhesion Assay to Study Leptospiral Proteins: An Approach to Investigate Host-Pathogen Interaction -- Complement Resistance Assays -- Evaluation of Intracellular Trafficking in Macrophages -- Biofilm Formation and Quantification using the 96-Microtiter Plate -- Survival Tests for Leptospira spp. -- Cultivation of Leptospira interrogans within Rat Peritoneal Dialysis Membrane Chambers -- Use of Golden Syrian Hamster as an Animal Model to Study Leptospirosis-Associated Immune Responses -- Evaluation of Vaccine Candidates against Leptospirosis using Golden Syrian Hamsters -- Leptospira and Leptospirosis -- Laboratory Diagnosis of Leptospirosis.
  • 일반주제명
    Microbiology.
    Infectious diseases.
    Microbiology
    Infectious Diseases
  • 기본자료 저록
    Springer Nature eBook
  • ISBN
    9781071604595
  • 언어
    영어

초록

This volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms.

목차

Isolation and Culture of Leptospira from Clinical and Environmental Samples
Core Genome Multi-Locus Sequence Typing Analyses of Leptospira spp. using the Bacterial Isolate Genome Sequence Database
Use of MALDI-ToF Mass Spectrometry for Identification of Leptospira
Cell Enumeration of Leptospira by Flow Cytometry
The Single-Step Method of RNA Purification Applied to Leptospira
Purification of LPS from Leptospira
Isolation, Purification, and Characterization of Leptophages
Creating a Library of Random Transposon Mutants in Leptospira
Transposon Sequencing in Leptospira interrogans
Specific Gene Silencing in Leptospira biflexa by RNA-Guided Catalytically inactive Cas9 (dCas9)
Leptospira spp. Toolbox for Chemotaxis Assay
In Situ Structural Analysis of Leptospira spp. by Electron Cryotomography
Measurement of the Cell-Body Rotation of Leptospira
In Vivo Imaging of Bioluminescent Leptospires
Cell Monolayer Translocation Assay
Cell Adhesion Assay to Study Leptospiral Proteins: An Approach to Investigate Host-Pathogen Interaction
Complement Resistance Assays
Evaluation of Intracellular Trafficking in Macrophages
Biofilm Formation and Quantification using the 96-Microtiter Plate
Survival Tests for Leptospira spp.
Cultivation of Leptospira interrogans within Rat Peritoneal Dialysis Membrane Chambers
Use of Golden Syrian Hamster as an Animal Model to Study Leptospirosis-Associated Immune Responses
Evaluation of Vaccine Candidates against Leptospirosis using Golden Syrian Hamsters
Leptospira and Leptospirosis
Laboratory Diagnosis of Leptospirosis