E-Book

RNA-Chromatin Interactions: Methods and Protocols [electronic resource]

  • Ørom, Ulf Andersson Vang. editor
  • Humana
  • 2020
RNA-Chromatin Interactions: Methods and Protocols [electronic resource]
  • 자료유형
    단행본
  • 서명/저자사항
    RNA-Chromatin Interactions:Methods and Protocols[electronic resource] / edited by Ulf Andersson Vang Ørom.
  • 판차
    1st ed. 2020.
  • 발행사항
    New York, NY : Humana, 2020.
  • 개인저자
    Ørom, Ulf Andersson Vang., editor
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    1 online resource.
  • 총서명
    Methods in Molecular Biology,1064-3745 ; 2161
  • 일반주제명
    Human genetics.
    Human Genetics
  • 기본자료 저록
    Springer Nature eBook
  • ISBN
    9781071606803
  • 언어
    영어

초록

This volume focuses on RNAs interacting with chromatin and their function. Chapters guide readers through transcription, splicing, non-coding RNA function and manipulation of gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA-Chromatin Interactions: Methods and Protocols aims to be a starting-point to expand researchers experimental approaches towards the numerous outstanding questions in this new and expanding field

목차

Tuning the Expression of Long Noncoding RNA loci with CRISPR Interference
Identification of Chromatin binding sites for Long noncoding RNAs by Chromatin Oligo Affinity Precipitation (ChOP)
Knock-down of Nuclear lncRNAs by Locked Nucleic Acid (LNA) Gapmers in Nephron Progenitor Cells
Design and Application of a Short (16-mer) Locked Nucleic Acid splice-Switching Oligonucleotide for Dystrophin Production in Duchenne Muscular Dystrophy Myotubes
Targeting Polyadenylation for Retention of RNA at Chromatin
Simultaneous Detection of RNAs and Proteins with Subcellular Resolution
In Vivo Crosslinking of Histone and RNA-binding Proteins
Proteomics-based Systematic Identification of Nuclear Proteins Anchored to Chromatin via RNA
2D Saturation Transfer Difference NMR for Determination of Protein Binding Sites on RNA Guanine Quadruplexes
Mapping Transcriptome-wide and Genome-wide RNA-DNA Contacts with Chromatin-Associated RNA Sequencing (ChAR-seq)
Identification of cis-elements for RNA Subcellular Localization through REL-seq
Transcriptome-wide Mapping of Protein-RNA Interactions
Proximity RNA-seq, A Sequencing Method to Identify Co-localization of RNA
Immunoprecipitation of DNA-RNA Hybrids using the S9.6 Antibody
Characterization of R-loop Structures using Single-Molecule R-loop Footprinting and Sequencing
Characterization of R-loop Structures using Single-Molecule R-loop Footprinting and Sequencing
Analyzing RNA-DNA Triplex Formation in Chromatin
Mapping RNA-chromatin interactions in vivo with RNA-DamID.