Arbuscular Mycorrhizal Fungi : Methods and Protocols [electronic resource]

  • Ferrol, Nuria. editor. , Lanfranco, Luisa. editor.
  • Springer US : Imprint: Humana
  • 2020
Arbuscular Mycorrhizal Fungi : Methods and Protocols [electronic resource]


This book compiles the most comprehensive collection of protocols currently used in arbuscular mycorrhizal (AM) fungal research. This experience-based collection includes methods for isolation, cultivation, detection, and quantification of AM fungi, as well as the use of metagenomics for community studies and experimental procedures for functional genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Arbuscular Mycorrhizal Fungi: Methods and Protocols serves as an ideal aid for researchers seeking to perform experiments that fill the gaps in our knowledge of the basic biology, functions, and ecology of AM fungi in the hope of deploying these powerful plant fungal symbionts in agriculture more effectively.


Isolation and Culture of Arbuscular Mycorrhizal Fungi from Field Samples / Dora Trejo-Aguilar, Jacob Banuelos
The Mycorrhizal Donor Plant (MDP) In Vitro Culture System for the Efficient Colonization of Whole Plants / Ismahen Lalaymia, Stéphane Declerck
A Whole-Plant Culture Method to Study Structural and Functional Traits of Extraradical Mycelium / Cristiana Sbrana, Alessandra Pepe, Nuria Ferrol, Manuela Giovannetti
Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization / Tania Ho-Plágaro, María Isabel Tamayo-Navarrete, José Manuel García-Garrido
Fluorescent Staining of Arbuscular Mycorrhizal Structures Using Wheat Germ Agglutinin (WGA) and Propidium Iodide / Gennaro Carotenuto, Andrea Genre
Visualization of Arbuscular Mycorrhizal Fungal Extraradical Hyphae and Spores Vitality and Activity / Pablo Cornejo, Humberto Aponte
Molecular Quantification of Arbuscular Mycorrhizal Fungal Root Colonization / Martina Janoušková, Petra Caklová
Single-Spore Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi / Dirk Redecker
High-Throughput DNA Sequence-Based Analysis of AMF Communities / Íris Marisa Maxaieie Victorino, Andrea Berruti, Alberto Orgiazzi, Samuele Voyron, Valeria Bianciotto, Erica Lumini
Analysis of Arbuscular Mycorrhizal Fungal Communities by Terminal Restriction Fragment Length Polymorphism (TRFLP) / Álvaro López-García
Bioinformatic Methods for the Analysis of High-Throughput RNA Sequencing in Arbuscular Mycorrhizal Fungi / Francesco Venice, Alessandra Salvioli di Fossalunga, Paola Bonfante
Arbuscular Mycorrhizal Fungal Gene Expression Analysis by Real-Time PCR / Alessandro Silvestri, Jacob Pérez-Tienda, Juan Antonio López-Ráez
Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis / Raffaella Balestrini, Valentina Fiorilli
Detection of Arbuscular Mycorrhizal Fungal Gene Expression by In Situ Hybridization / Bettina Hause, Natalia Requena
Functional Analysis of Arbuscular Mycorrhizal Fungal Genes in Yeast / Elisabeth Tamayo, Tamara Gómez-Gallego, Nuria Ferrol
Isotope Labeling to Study Phosphorus Uptake in the Arbuscular Mycorrhizal Symbiosis / Carla Cruz-Paredes, Mayra E. Gavito
Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycorrhizae / Ghislaine Recorbet, Pierre-Emmanuel Courty, Daniel Wipf
Host-Induced Gene Silencing of Arbuscular Mycorrhizal Fungal Genes via Agrobacterium rhizogenes-Mediated Root Transformation in Medicago truncatula / Meike Hartmann, Stefanie Voß, Natalia Requena
Application of Virus-Induced Gene Silencing to Arbuscular Mycorrhizal Fungi / Tatsuhiro Ezawa, Hayato Maruyama, Yusuke Kikuchi, Kaede Yokoyama, Chikara Masuta