Brain development: methods and protocols [electronic resource]

  • Sprecher, Simon G.
  • Humana Press
  • 2020
Brain development: methods and protocols [electronic resource]


This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Invertebrate Models
Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra / Wanda Buzgariu, Marie-Laure Curchod, Chrystelle Perruchoud, Brigitte Galliot
Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis / Jamie A. Havrilak, Michael J. Layden
Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis / Fabian Rentzsch, Eduard Renfer, Ulrich Technau
Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System / Elena Perea-Atienza, Brenda Gavilán, Simon G. Sprecher, Pedro Martinez
Immunostaining of the Embryonic and Larval Drosophila Brain / Frank Hirth, Danielle C. Diaper
Nonfluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila / David Jussen, Rolf Urbach
Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling / Karoline F. Kraft, Rolf Urbach
Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update / Emma L. Powell, Iris Salecker
Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain / Karolina Miszczak, Boris Egger
CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila / Cornelia Fritsch, Simon G. Sprecher
The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development / Max S. Farnworth, Kolja N. Eckermann, Hassan M. M. Ahmed, Dominik S. Mühlen, Bicheng He, Gregor Bucher
A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum / Marita Buescher, Georg Oberhofer, Natalia Carolina Garcia-Perez, Gregor Bucher
Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium / Vera S. Hunnekuhl, Janna Siemanowski, Max S. Farnworth, Bicheng He, Gregor Bucher
X-Ray Microscopy of the Larval Crustacean Brain / Jakob Krieger, Franziska Spitzner
Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain / Steffen Harzsch, Caroline Viertel
Immunostainings in Nervous System Development of the Nematode C. elegans / Janet S. Duerr
Methods in Brain Development of Molluscs / Andreas Wanninger, Tim Wollesen
A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization / Clare Hudson
Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum / Yann Le Petillon, Stéphanie Bertrand, Héctor Escrivà

Vertebrate Models
In Situ Hybridization and Immunostaining of Xenopus Brain / Kai-li Liu, Xiu-mei Wang, Zi-long Li, Ying Liu, Rong-qiao He
Morpholino Studies in Xenopus Brain Development / Jennifer E. Bestman, Hollis T. Cline
Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish / Gilbert Lauter, Iris Söll, Giselbert Hauptmann
Live Morphometric Classification of Sensory Neurons in Larval Zebrafish / Gema Valera, Hernán López-Schier
Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain / Richard P. Tucker, Tatsuto Ishimaru, Qizhi Gong
Gene Silencing in Chicken Brain Development / Georgia Tsapara, Irwin Andermatt, Esther T. Stoeckli
Transplantation of Neural Tissue: Quail–Chick Chimeras / Andrea Streit, Claudio D. Stern
Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development / Jinling Liu, Aimin Liu
The Cre/Lox System to Assess the Development of the Mouse Brain / Claudius F. Kratochwil, Filippo M. Rijli
In Utero Electroporation to Study Mouse Brain Development / Emilie Pacary, François Guillemot