Molecular Dermatology : Methods and Protocols [electronic resource]

  • Botchkareva, Natalia V. editor. , Westgate, Gillian E. editor.
  • Springer US : Imprint: Humana
  • 2020
Molecular Dermatology : Methods and Protocols [electronic resource]


This volume delivers the most important laboratory techniques that underlie dermatological investigation, including in situ, in vitro, ex vivo, and in vivo models of skin, its appendages (hair follicles, sebaceous and sweat glands), and immune cells. Also included are unique techniques that unravel epigenetic mechanisms in skin and hair follicle cells, such as gene organization in the nucleus and gene expression regulation by the non-coding transcriptome, which are important recent developments in investigative dermatology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Molecular Dermatology: Methods and Protocols serves as an essential resource for researchers working in the areas of fundamental as well as applied dermatological science.


Isolation of Epidermal Keratinocytes from Human Skin: The Scratch-Wound Assay for Assessment of Epidermal Keratinocyte Migration / Irene Castellano-Pellicena, M. Julie Thornton
Isolation of Different Dermal Fibroblast Populations from the Skin and the Hair Follicle / Rachael Williams, M. Julie Thornton
Isolation of Epidermal and Hair Follicle Melanocytes / Richard Baker, M. Julie Thornton
Isolation and Culture of Human Skin Mast Cells / Hanna Siiskonen, Jörg Scheffel
Organotypic 3D Skin Models: Human Epidermal Equivalent Cultures from Primary Keratinocytes and Immortalized Keratinocyte Cell Lines / Gijs Rikken, Hanna Niehues, Ellen H. van den Bogaard
Purification of Extracellular Microvesicles Secreted by Dermal Fibroblasts / Isabelle Petit, Ayelet Levy, Daniel Aberdam
A Method to Investigate the Epidermal Permeability Barrier In Vitro / Yoshikazu Uchida, Anna Celli
Isolating Dermal Papilla Cells from Human Hair Follicles Using Microdissection and Enzyme Digestion / Summik Limbu, Claire A. Higgins
Methods to Study Human Hair Follicle Growth Ex Vivo: Human Microdissected Hair Follicle and Human Full Thickness Skin Organ Culture / Janin Edelkamp, Jennifer Gherardini, Marta Bertolini
Nonsurgical Induction of Alopecia Areata in C3H/HeJ Mice via Adoptive Transfer of Cultured Lymphoid Cells / Eddy H. C. Wang, Kevin J. McElwee
Collapse and Restoration of Hair Follicle Immune Privilege Ex Vivo: A Model for Alopecia Areata / Markus Fehrholz, Marta Bertolini
Experimentally Induced Epithelial–Mesenchymal Transition of Human Hair FollicleStem Cells as a Model of Scarring Alopecia Ex Vivo / Ilaria Piccini, Marta Bertolini
Generation of Hair Follicle Germs In Vitro Using Human Postnatal Skin Cells / Ekaterina P. Kalabusheva, Ekaterina A. Vorotelyak
Method for Human Eccrine Sweat Gland Isolation from the Scalp by Means of the Micropunch Technique and Vital Dyes / Francisco Jiménez
Identification of Long Noncoding RNA by In Situ Hybridization Approaches / Mara Mancini, Anna Maria Lena, Eleonora Candi
Detection of MicroRNAs by In Situ Hybridization in Skin / Maximilian E. Pickup, Mohammed I. Ahmed
Chromatin Immunoprecipitation of Low Number of FACS-Purified Epidermal Cells / Carmit Bar, V. Julian Valdes, Elena Ezhkova
3D-FISH Analysis of the Spatial Genome Organization in Skin Cells in Situ / Andrei N. Mardaryev, Michael Y. Fessing
Method to Study Skin Cancer: Two-Stage Chemically Induced Carcinogenesis in Mouse Skin / Andrei N. Mardaryev
Investigation of Skin Wound Healing Using a Mouse Model / Jianmin Wu, Ning Xu Landén
Human Wound Healing Ex Vivo Model with Focus on Molecular Markers / Jennifer Gherardini, Max van Lessen, Ilaria Piccini, Janin Edelkamp, Marta Bertolini
Method for Investigation of Photobiological Effects of Light on Human Skin Cells Mediated by Low Doses of Light / Charles Mignon, Natallia E. Uzunbajakava