Single Molecule Microscopy in Neurobiology [electronic resource]

  • Yamamoto, Nobuhiko. editor. , Okada, Yasushi. editor.
  • Springer US : Imprint: Humana
  • 2020
Single Molecule Microscopy in Neurobiology [electronic resource]


This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field.


Single-Molecule Imaging of Intracellular Transport in Neurons and Non-neuronal Cells: From Microscope Optics to Sample Preparations / Jay Yuan Jian Wang, Taketoshi Kambara, Yasushi Okada
In Vivo Single-Molecule Tracking of Voltage-Gated Calcium Channels with Split-Fluorescent Proteins in CRISPR-Engineered C. elegans / Yunke Zhao, Fabien Pinaud
Nanocores and Liquid Droplets: Single-Molecule Microscopy of Neuronal Stress Granule Components / Benedikt Niewidok, Rainer Kurre, Roland Brandt
Live-Cell Single-Molecule Imaging with Optogenetics Reveals Dynamics of a Neuronal Activity-Dependent Transcription Factor / Hironobu Kitagawa, Noriyuki Sugo, Nobuhiko Yamamoto
Single-Molecule Imaging of Recycling Synaptic Vesicles in Live Neurons / Merja Joensuu, Ramon Martínez-Mármol, Mahdie Mollazade, Pranesh Padmanabhan, Frédéric A. Meunier
Approaching Protein-Protein Interactions in Membranes Using Single-Particle Tracking and Packing Coefficient Analysis / Marianne Renner, Antoine Triller
Synaptic Function and Neuropathological Disease Revealed by Quantum Dot-Single-Particle Tracking / Hiroko Bannai, Takafumi Inoue, Matsumi Hirose, Fumihiro Niwa, Katsuhiko Mikoshiba
Multipolarization Dark-Field Imaging of Single Endosomes in Microfluidic Neuronal Culture for Simultaneous Orientation and Displacement Tracking / Luke Kaplan, Bianxiao Cui
Practical Guidelines for Two-Color SMLM of Synaptic Proteins in Cultured Neurons / Xiaojuan Yang, Christian G. Specht
Single-Molecule Localization Microscopy Propelled by Small Organic Fluorophores with Blinking Properties / Akihiko Morozumi, Mako Kamiya, Yasuteru Urano
Highly Biocompatible Super-resolution Imaging: SPoD-OnSPAN / Tetsuichi Wazawa, Takashi Washio, Takeharu Nagai
Nanoscale Molecular Imaging of Presynaptic Active Zone Proteins in Cultured Hippocampal Neurons / Hirokazu Sakamoto, Shigeyuki Namiki, Kenzo Hirose
Three-Dimensional Super-Resolution Imaging of the Cytoskeleton in Hippocampal Neurons Using Selective Plane Illumination / Frances Camille M. Wu, Feby Wijaya Pratiwi, Chin-Yi Chen, Chieh-Han Lu, Wei-Chun Tang, Yen-Ting Liu et al.
A Protocol for Single-Molecule Translation Imaging in Xenopus Retinal Ganglion Cells / Florian Ströhl, Julie Qiaojin Lin, Francesca W. van Tartwijk, Hovy Ho-Wai Wong, Christine E. Holt, Clemens F. Kaminski
Investigating Molecular Diffusion Inside Small Neuronal Compartments with Two-Photon Fluorescence Correlation Spectroscopy / Kazuki Obashi, Shigeo Okabe