Zika Virus: Methods and Protocols [electronic resource]

  • Kobinger, Gary. editor. , Racine, Trina. editor.
  • Springer US : Imprint: Humana
  • 2020
Zika Virus: Methods and Protocols [electronic resource]


This volume discusses the latest protocols used by scientists to understand ZIKV and how to mitigate its presence. These studies will benefit in the detection, diagnosis, management, treatment, and prevention of this infection. The chapters in this book cover techniques in virus isolation, purification, and ways to titrate the virus; recent advancements made in the development of modified molecular techniques; and a discussion on Zika virus itself to help readers better understand this pathogen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Zika Virus: Methods and Protocols is a valuable resource for both novice and expert scientists interested in learning more about this important topic.


Zika Virus: A Brief History and Review of Its Pathogenesis Rediscovered / Joel N. Maslow, Christine C. Roberts
Zika Virus Isolation, Purification, and Titration / Mariana Baz
Evaluating Zika Virus Pathogenesis in Immunocompromised Mice / Bryan D. Griffin, David Safronetz, Darwyn Kobasa
Droplet Digital PCR and Immunohistochemistry Techniques to Detect Zika Virus in the Central Nervous System of Mice / William Enlow, Jocelyne Piret, Guy Boivin
A Plaque Reduction Neutralization Test for the Detection of ZIKV-Specific Antibodies / Kristina Dimitrova, Emelissa J. Mendoza, Nicole Mueller, Heidi Wood
Analysis of Serum Anti-Zika Virus Antibodies by Focus Reduction Neutralization Test / Jessica L. Smith, Alec J. Hirsch
Enzyme-Linked Immunosorbent Assay and Quantitative Reverse Transcription PCR as a Technique to Analyze Inflammation / Raquel das Neves Almeida, Heloisa Antoniella Braz-de-Melo, Rafael Corrêa, Kelly Grace Magalhães
Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells / Young Chan Kim, Nallely Garcia-Larragoiti, Cesar Lopez-Camacho, Martha Eva Viveros-Sandoval, Arturo Reyes-Sandoval
Optimization of Small-Scale Production of Zika Virus Envelope Glycoprotein by Transient Expression in HEK293 Cells for ELISA / Young Chan Kim, Maud Dumoux, Raymond J. Owens, Arturo Reyes-Sandoval
Zika Virus Peptide ELISA (ZIKV-NS2B-Concat ELISA) for Detection of IgG Antibodies to Zika Virus Infection / Nischay Mishra, Riddhi Thakkar, James Ng, W. Ian Lipkin
Zika Virus Amplification Using Strand Displacement Isothermal Method and Sequencing Using Nanopore Technology / Sören Hansen, Oumar Faye, Sabri S. Sanabani, Martin Faye, Susanne Böhlken-Fascher, Ousmane Faye et al.
Detection of Zika Virus Using Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) / Laura E. Lamb, Sarah N. Bartolone, Michael B. Chancellor
Development and Validation of Real-Time RT-LAMP Assays for the Specific Detection of Zika Virus / Benjamin Lopez-Jimena, Mohammed Bakheit, Michaël Bekaert, Graham Harold, Sieghard Frischmann, Cheikh Fall et al.
Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA / Cheikh Tidiane Diagne, Martin Faye, Benjamin Lopez-Jimena, Ahmed Abd El Wahed, Cheikh Loucoubar, Cheikh Fall et al.
A Porcine Model of Zika Virus Infection to Profile the In Utero Interferon Alpha Response / Ivan Trus, Stewart Walker, Maria Fuchs, Daniel Udenze, Volker Gerdts, Uladzimir Karniychuk
Isolation and Detection of Zika Virus-Infected Rhesus Macaques Lymph Node Cells and Splenocytes / Nicole Haese, Alec J. Hirsch, Daniel N. Streblow
Discovery of Zika Virus Dependency and Restriction Factors Using Flow-Based Arrayed CRISPR Screening for Identification of Targets (FACS-IT) / William M. McDougall, Manish Kandpal, Jill M. Perreira, Abraham L. Brass
Zika Virus Targets Multiple Tissues and Cell Types During the First Trimester of Pregnancy / Nabila Jabrane-Ferrat, Francisco Veas
Use of Primary Human Fetal Astrocytes and Tissue Explants as Ex Vivo Models to Study Zika Virus Infection of the Developing Brain / Daniel Limonta, William Branton, Cheung Pang Wong, Leina Saito, Christopher Power, Tom C. Hobman
Isolation of Monoclonal Antibodies from Zika Virus-Infected Patient Samples / Qihui Wang, Jinghua Yan