Bioluminescent imaging : methods and protocols [electronic resource]

  • Ripp, Steven
  • Humana Press
  • 2020
Bioluminescent imaging : methods and protocols [electronic resource]


This volume provides detailed prools and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.


Part I: In Vitro/Ex Vivo Imaging Applications
1. High-throughput Bioluminescence Imaging and Reporter Gene Assay with 3D Spheroids from Human Cell LinesMaria Maddalena Calabretta, Laura Montali, Antonia Lopreside, Elisa Michelini, and Aldo Roda
2. Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells Setsuko Tsuboi and Takashi Jin
3. High-throughput Analysis of Endocrine Disrupting Compounds using BLYES and BLYAS Bioluminescent Yeast Bioassays Tingting Xu, Anna Young, Jasleen Narula, Gary Sayler, and Steven Ripp
4. Bioluminescent Imaging of Single Bacterial Cells Using an Enhanced ilux OperonCarola Gregor
5. Bioluminescent Imaging and Tracking of Bacterial Transport in SoilsJie Zhuang, Weipeng Liu, Liqiong Yang, Jia Kang, and Xiaoming Zhang

Part II: In Vivo Imaging of Microbial Pathogenesis
6. In vivo Bioluminescent Imaging of Yersinia ruckeri Pathogenesis in FishJose A. Guijarro and Jessica Mendez
7. In Vivo Bioluminescent Monitoring of Parasites in BALB/c Mouse Models of Cutaneous Leishmaniasis Drug DiscoveryDiana Caridha, Susan Leed, and Alicia Cawlfield
8. Multiplex Imaging of Polymicrobial Communities - Murine Models to Study Oral Microbiome InteractionsJens Kreth, Yasser M. Abdelrahman, and Justin Merritt
9. Bioluminescence Imaging to Study Mature Biofilm Formation by Candida spp. and Antifungal Activity in vitro and in vivoKatrien Van Dyck , Patrick Van Dijck, and Greetje Vande Velde

Part III: In Vivo Small Animal/Organism Imaging Applications
10. Bioluminescence Imaging of Neuroinflammation in a Mouse Model of Parkinson's DiseaseMaria Eugenia Bernis and Gultekin Tamguney
11. Continual Conscious Bioluminescent Imaging in Freely Moving Mice Juan Antinao Diaz, Amy Geard, Lorna M. FitzPatrick, Juliette M.K.M Delhove, Suzanne M.K. Buckley, Simon N. Waddington, Tristan R. McKay, and Rajvinder Karda
12. In Vivo Bioluminescent Imaging of Marburg Virus in a Rodent ModelShan Lei, Weijin Huang, Youchun Wang, and Qiang Liu
13. Continuous and Real-time In Vivo Autobioluminescent Imaging in a Mouse ModelDerek Yip, Andrew Kirkpatrick, Tingting Xu, Tom Masi, Stacy Stephenson, Steven Ripp, and Dan Close
14. In vivo Tracking of Tumor-derived Bioluminescent Extracellular Vesicles in MicePrakash Gangadaran and Byeong-Cheol Ahn
15. Bioluminescence Imaging in the Chick Chorioallantoic Membrane AssayBenedikt Jefferies, Zhichao Tong, and Roman Nawroth
16. Application of a cybLuc Aminoluciferin for Deep Tissue Bioluminescence Imaging in Rodent Models Xiang Li and Minyong Li

Part IV: In Planta Imaging Applications
17. The Application of Single Cell Bioluminescent Imaging to Monitor Circadian Rhythms of Individual Plant CellsTomoaki Muranaka and Tokitaka Oyama