E-Book

Caveolae: Methods and Protocols [electronic resource]

  • Blouin, Cedric M. editor
  • Springer US : Imprint: Humana
  • 2020
Caveolae: Methods and Protocols [electronic resource]
  • 자료유형
    단행본
  • 서명/저자사항
    Caveolae:Methods and Protocols[electronic resource] / edited by Cedric M. Blouin.
  • 판차
    1st ed. 2020.
  • 발행사항
    New York, NY : Springer US : Imprint: Humana, 2020.
  • 개인저자
    Blouin, Cedric M., editor
  • 단체저자
    SpringerLink (Online service)
  • 형태사항
    XI, 218 p. 46 illus., 37 illus. in color. .
  • 총서명
    Methods in Molecular Biology,1064-3745 ; 2169
  • 내용주기
    Selective Visualization of Caveolae by TEM using APEX2 -- Freeze-Fracture Replica Immunolabeling of Cryopreserved Membrane Compartments, Cultured Cells and Tissues -- Analysis of Caveolin in Primary Cilia -- Method for Efficient Observation of Caveolin-1 Plasma Membrane by Microscopy Imaging Analysis -- Quantitative Image Analysis of the Spatial Organization and Mobility of Caveolin Aggregates at the Plasma Membrane -- Spatiotemporal Analysis of Caveolae Dynamics using Total Internal Reflection Fluorescence Microscopy -- Live-Cell FRET Imaging of Phosphorylation-Dependent Caveolin-1 Switch -- GPMVs as a Tool to Study Caveolin-Interacting Partners -- Biotin Proximity Labeling to Identify Protein-Protein Interactions for Cavin1 -- Investigation of Novel Cavin-1/Suppressor of Cytokine 3 (SOCS3) Interactions by Co-Immunoprecipitation, Peptide Pull-Down and Peptide Array Overlay Approaches -- Analysis of Protein and Lipid Interactions using Liposome Co-Sedimentation Assays -- Liposome Binding Assay to Characterize Cavin Family Protein Structure and Functions -- Preparation of Caveolin-1 for NMR Spectroscopy Experiments -- Tagging and Deleting of Endogenous Caveolar Components using CRISPR/Cas9 Technology -- Pulling of Tethers from the Cell Plasma Membrane using Optical Tweezers -- Live Confocal Imaging of Zebrafish Notochord Cells under Mechanical Stress In Vivo -- Study of Caveolae Dependent Mechanoprotection in Huma Muscle Cells using Micropatterning and Live Cell Microscopy -- Immunofluorescence-Based Analysis of Caveolin-3 in the Diagnostic Management of Neuromuscular Diseases.
  • 일반주제명
    Lipids.
    Cell biology.
    Lipidology
    Cell Biology
  • 기본자료 저록
    Springer eBooks
  • 기타형태 저록
    Printed edition: 9781071607312
  • ISBN
    9781071607329
  • 언어
    영어

초록

This volume explores techniques used to study either the structure or the functions of caveolae and their components in several normal and pathophysiological situations. The chapters in this book cover topics such as selective visualization of caveolae by electron microscopy techniques; spatiotemporal analysis of caveolae dynamics and mechanics using live cell fluorescence microscopy; in vitro reconstitution such as liposomes and GPMVs as tools to study caveolin- and cavin-interacting partners; pulling of tethers from the cell plasma membrane using optical tweezers; and immunofluorescence-based analysis of caveolin-3 in the diagnostic management of neuromuscular diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Caveolae: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in discovering new roles or regulations of formed caveolae, and proteins composing their coat in various model organisms

목차

Selective Visualization of Caveolae by TEM Using APEX2 / Alexander Ludwig
Freeze-Fracture Replica Immunolabeling of Cryopreserved Membrane Compartments, Cultured Cells and Tissues / Eric Seemann, Michael M. Kessels, Britta Qualmann
Analysis of Caveolin in Primary Cilia / Stine K. Morthorst, Johanne B. Mogensen, Søren T. Christensen, Lotte B. Pedersen
Method for Efficient Observation of Caveolin-1 in Plasma Membrane by Microscopy Imaging Analysis / Tomoya Yamaguchi, Toyoshi Fujimoto, Takashi Takahashi
Quantitative Image Analysis of the Spatial Organization and Mobility of Caveolin Aggregates at the Plasma Membrane / Takashi Hirama, Raibatak Das
Spatiotemporal Analysis of Caveolae Dynamics Using Total Internal Reflection Fluorescence Microscopy / Yosuke Senju, Shiro Suetsugu
Live-Cell FRET Imaging of Phosphorylation-Dependent Caveolin-1 Switch / Adriana M. Zimnicka, Zhenlong Chen, Peter T. Toth, Richard D. Minshall
GPMVs as a Tool to Study Caveolin-Interacting Partners / Joanna Podkalicka, Cedric M. Blouin
Biotin Proximity Labeling to Identify Protein–Protein Interactions for Cavin1 / Carolina Mendoza-Topaz
Investigation of Novel Cavin-1/Suppressor of Cytokine Signaling 3 (SOCS3) Interactions by Coimmunoprecipitation, Peptide Pull-Down, and Peptide Array Overlay Approaches / Jamie J. L. Williams, George S. Baillie, Timothy M. Palmer
Analysis of Protein and Lipid Interactions Using Liposome Co-sedimentation Assays / Elin Larsson, Madlen Hubert, Richard Lundmark
Liposome Binding Assay to Characterize the Structure and Function of Cavin Proteins / Miriam Stoeber
Preparation of Caveolin-1 for NMR Spectroscopy Experiments / Sarah M. Plucinsky, Jeffrey A. Julien, Kerney Jebrell Glover
Tagging and Deleting of Endogenous Caveolar Components Using CRISPR/Cas9 Technology / Elena Shvets, Carolina Mendoza-Topaz
Pulling of Tethers from the Cell Plasma Membrane Using Optical Tweezers / Darius V. Köster
Live Confocal Imaging of Zebrafish Notochord Cells Under Mechanical Stress In Vivo / Ye-Wheen Lim, Harriet P. Lo, Thomas E. Hall, Robert G. Parton
Study of Caveolae-Dependent Mechanoprotection in Human Muscle Cells Using Micropatterning and Live-Cell Microscopy / Melissa Dewulf, Cedric M. Blouin
Immunofluorescence-Based Analysis of Caveolin-3 in the Diagnostic Management of Neuromuscular Diseases / Andreas Roos, Denisa Hathazi, Ulrike Schara