DNA-Protein Interactions Principles and Protocols [electronic resource] :

  • Leblanc, Benoit P. editor. , Rodrigue, Sebastien. editor.
  • 2015
DNA-Protein Interactions Principles and Protocols [electronic resource] :
  • Data Type
  • Title/Author
    DNA-Protein Interactions[electronic resource] :Principles and Protocols / edited by Benoit P. Leblanc, Sebastien Rodrigue.
  • Edition
    4th ed. 2015.
  • Author
    Leblanc, Benoit P., editor., Rodrigue, Sebastien., editor.
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    XI, 347 p. 73 illus., 24 illus. in color. : online resource.
  • Total State.
    Methods in Molecular Biology,1064-3745 ; 1334
  • Contents Note
    Electrophoretic Mobility Shift Assay Using Radiolabeled DNA Probes -- In Vitro DNase I Footprinting -- Determining the Architecture of a Protein/DNA Complex by Combining FeBABE Cleavage Analyses, 3-D Printed Structures, and the ICM Molsoft Program -- In Cellulo DNA Analysis: LMPCR Footprinting -- Southwestern Blotting Assay -- Single-Molecule Approaches for the Characterization of Riboswitch Folding Mechanisms -- Probing of Nascent Riboswitch Transcripts -- Functional Studies of DNA-Protein Interactions Using FRET Techniques -- Precise Identification of Genome-Wide Transcription Start Sites in Bacteria by 5' Rapid Amplification of cDNA Ends (5'-RACE) -- Analysis of DNA Supercoiling Induced by DNA-Protein Interactions -- Precise Identification of DNA-Binding Proteins Genomic Location by Exonuclease Coupled Chromatin Immunoprecipitation (ChIP-exo) -- The Cruciform DNA Mobility Shift Assay: A Tool to Study Proteins that Recognize Bent DNA -- Individual and Sequential Chromatin Immunoprecipitation Protocols -- Chromatin Endogenous Cleavage (ChEC) as a Method to Quantify Protein Interaction with Genomic DNA in Saccharomyces cerevisiae -- Selection and Validation of Spacer Sequences for CRISPR-Cas9 Genome Editing and Transcription Regulation in Bacteria -- Detection of Short-Range DNA Interactions in Mammalian Cells Using High-Resolution Circular Chromosome Conformation Capture Coupled to Deep Sequencing -- Global Mapping of Open Chromatin Regulatory Elements by Formaldehyde-Assisted Isolation of Regulatory Elements Followed by Sequencing (FAIRE-seq) -- Aggregate and Heatmap Representations of Genome-Wide Localization Data Using VAP, a Versatile Aggregate Profiler -- Circular Dichroism for the Analysis of Protein-DNA Interactions -- Quantitative Investigation of Protein-Nucleic Acid Interactions by Biosensor Surface Plasmon Resonance -- Identification of Nucleic Acid High Affinity Binding Sequences of Proteins by SELEX.
  • Topical Term
    Human genetics.
    Human Genetics.
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9781493928767
  • ISBN
  • Language