Germinal Centers Methods and Protocols [electronic resource] :

  • Calado, Dinis Pedro. editor.
  • 2017
Germinal Centers Methods and Protocols [electronic resource] :
  • Data Type
  • Title/Author
    Germinal Centers[electronic resource] :Methods and Protocols / edited by Dinis Pedro Calado.
  • Author
    Calado, Dinis Pedro., editor.
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    XI, 338 p. 64 illus., 49 illus. in color. : online resource.
  • Total State.
    Methods in Molecular Biology,1064-3745 ; 1623
  • Contents Note
    Analysis of the Germinal Center Reaction in Tissue Sections -- Detecting Gene Expression in Lymphoid Microenvironments by Laser Microdissection and Quantitative RT-PCR -- Tracking Plasma Cell Differentiation in Living Mice with 2-Photon Microscopy -- Microanatomical Labeling of Germinal Center Structures for Flow Cytometry Using Photoactivation -- Fate Mapping and Transcript Profiling of Germinal Center Cells by Two-photon Photoconversion -- Intravital Microscopy of T-B Cell Interactions in Germinal Centers -- Identifying Follicular Regulatory T cells by Confocal Microscopy -- Cytokine Expression by T follicular Helper Cells -- Follicular Dendritic Cell Isolation and Loading of Immune Complexes -- Isolation and Characterization of Mouse and Human Follicular Dendritic Cells -- In-vitro-induced Germinal Center B Cell Culture System -- CRISPR/Cas9 Mediated In Vitro Mutagenesis in GC-like B Cells -- Germinal Center Formation with Retrovirally-transduced B Cells for Determining the Role of Specific Molecules In Vivo -- Characterization of the B Cell Transcriptome by RNA-Binding Proteins using iCLIP -- Flow-Cytometric Method Measuring B Cell Surface Immunoglobulin Avidity -- Somatic Hypermutation and Affinity Maturation Analysis Using the 4-hydroxy-3-nitrophenyl-acetyl (NP) System -- Targeting Gene Function in Germinal Center B Cells: A Practical Approach -- Development of Mouse Model Systems of Germinal Centre Lymphomas -- The AID-Cre-ERT2 Model: A Tool for Monitoring B Cell Immune Responses and Generating Selective Hybridomas -- Determining the Origin of Human Germinal Center B Cell-derived Malignancies -- Tracking B-cell Repertoires and Clonal Histories in Normal and Malignant Lymphocytes -- How to Simulate a Germinal Centre. .
  • Topical Term
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9781493970940
  • ISBN
  • Language