Prions Methods and Protocols [electronic resource] :

  • Lawson, Victoria A. editor.
  • 2017
Prions Methods and Protocols [electronic resource] :
  • Data Type
  • Title/Author
    Prions[electronic resource] :Methods and Protocols / edited by Victoria A. Lawson.
  • Author
    Lawson, Victoria A., editor.
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    XI, 371 p. 52 illus., 26 illus. in color. : online resource.
  • Total State.
    Methods in Molecular Biology,1064-3745 ; 1658
  • Contents Note
    Purification and Fibrillation of Full-length Recombinant PrP -- Method for Folding of Recombinant Prion Protein to Soluble b-sheet Secondary Structure -- Analysis of Prion Protein Conformation using Circular Dichroism Spectroscopy -- Analysis of Prion Protein Structure using Nuclear Magnetic Resonance Spectroscopy -- Immunodetection of PrPSc혻using Western Immunoblotting Techniques -- Analysis of miRNA Signatures in Neurodegenerative Prion Disease -- Expression of Heterologous PrP and Prion Propagation in RK13 Cells -- Generation of Infectious Prions and Detection with the Prion Infected Cell Assay -- Analysis of Cellular Prion Protein Endoproteolytic Processing -- Cellular Analysis Adult Neural Stem Cells for Investigating Prion Biology -- Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons -- Methods of Protein Misfolding Cyclic Amplification -- RT-QuIC Assays for Prion Disease Detection and Diagnostics -- A Quick Method to Evaluate the Effect of the Amino Acid Sequence in the Misfolding Proneness of the Prion Protein -- Insights into Mechanisms of Transmission and Pathogenesis from Transgenic Mouse Models of Prion Diseases.-혻In vivo혻Near Infrared Imaging of Neurodegeneration -- Strain Typing of Prion Diseases using혻in vivo혻Mouse Models -- Preparation and Immunostaining of the Myenteric Plexus of Prion Infected Mice -- Cell Culture Methods for Screening of Prion Therapeutics -- Real-Time Quaking Induced Conversion for Diagnosis of Prion Disease -- Methods for Molecular Diagnosis of Human Prion Disease -- Molecular sub-typing of PrPres혻in Human Sporadic CJD Brain Tissue -- Intercellular Prion-like Conversion and Transmission of Cu/Zn Superoxide Dismutase (SOD1) in Cell Culture.
  • Topical Term
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9781493972425
  • ISBN
  • Language