Chromosomal Mutagenesis [electronic resource]

  • Pruett-Miller, Shondra M. editor.
  • 2015
Chromosomal Mutagenesis [electronic resource]
  • Data Type
  • Title/Author
    Chromosomal Mutagenesis[electronic resource] / edited by Shondra M. Pruett-Miller.
  • Edition
    2nd ed. 2015.
  • Author
    Pruett-Miller, Shondra M., editor.
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    XIV, 333 p. 22 illus. in color. : online resource.
  • Total State.
    Methods in Molecular Biology, Methods and Protocols,1064-3745 ; 1239
  • Contents Note
    Genome Editing by Targeted Chromosomal Mutagenesis -- 짯piggyBac Transposon-Based Insertional Mutagenesis in Mouse Haploid Embryonic Stem Cells -- Using Phage Integrases in a Site-Specific Dual Integrase Cassette Exchange Strategy -- Genome Mutagenesis Using Synthetic Triplex-Forming Molecules and Recombinagenic DNA -- Genome Engineering Using Adeno-Associated Virus (AAV) -- Engineering of Customized Meganucleases Via In Vitro Compartmentalization and In Cellulo Optimization -- Efficient Design and Assembly of Custom TALENs Using the Golden Gate Platform -- Ligation-Independent Cloning (LIC) Assembly of TALENs -- Assembly and Characterization of megaTALs for Hyperspecific Genome Engineering Applications -- Genome Engineering Using CRISPR-Cas9 System -- Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases -- Endogenous Gene Tagging with Fluorescent Proteins -- Silencing Long Non-Coding RNAs with Genome-Editing Tools -- Gene Editing Using ssODNs with Engineered Endonucleases -- Genome Editing in Human Pluripotent Stem Cells Using Site-Specific Nucleases -- Strategies to Increase Genome Editing Frequencies and to Facilitate the Identification of Edited Cells -- Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models -- Creating Knockout and Knockin Rodents Using Engineered Endonucleases Via Direct Embryo Injection -- Simple Sperm Preservation by Freeze-Drying for Conserving Animal Strains.
  • Topical Term
    Human genetics.
    Genetic engineering.
    Human Genetics.
    Genetic Engineering.
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9781493918614
  • ISBN
  • Language