miRNA Maturation Methods and Protocols [electronic resource] :

  • Arenz, Christoph. editor.
  • 2014
miRNA Maturation Methods and Protocols [electronic resource] :
  • Data Type
  • Title/Author
    miRNA Maturation[electronic resource] :Methods and Protocols / edited by Christoph Arenz.
  • Author
    Arenz, Christoph., editor.
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    X, 192 p. 50 illus., 29 illus. in color. : online resource.
  • Total State.
    Methods in Molecular Biology, Methods and Protocols,1064-3745 ; 1095
  • Contents Note
    The Pathway of miRNA Maturation -- Micro RNA Maturation and Human Disease -- Approaches to the Modulation of miRNA Maturation -- Expression Profiling of Components of the miRNA Maturation Machinery -- Primary microRNA Processing Assay Reconstituted using Recombinant Drosha and DGCR8 -- In-vivo processing Assay Based on a Dual Luciferase Reporter System to Evaluate DROSHA Enzymatic Activity -- Assaying Dicer-mediated miRNA Maturation by Means of Fluorescent Substrates -- A Fluorescence Correlation Spectroscopy-based Enzyme Assay for Human Dicer -- Detection of Micro RNA Maturation Using Unmodified pre-miRNA and Branched Rolling Circle Amplification -- Identification of Inhibitors of microRNA Function from Small Molecule Screens -- Quantitative RT-PCR Specific for Precursor and Mature miRNAs -- Cellular MicroRNA Sensors Based on Luciferase Reporters -- Inhibition of miRNA maturation by Peptide Nucleic Acids -- Molecular Methods for Validation of the Biological Activity of Peptide Nucleic Acids Targeting MicroRNAs -- Lentiviral Overexpression of miRNAs.
  • Topical Term
    Human genetics.
    Gene expression.
    Human Genetics.
    Gene Expression.
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9781627037020
  • ISBN
  • Language