PIWI-Interacting RNAs Methods and Protocols [electronic resource] :

  • Siomi, Mikiko C. editor.
  • 2014
PIWI-Interacting RNAs Methods and Protocols [electronic resource] :
  • Data Type
  • Title/Author
    PIWI-Interacting RNAs[electronic resource] :Methods and Protocols / edited by Mikiko C. Siomi.
  • Author
    Siomi, Mikiko C., editor.
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    XI, 249 p. 48 illus., 23 illus. in color. : online resource.
  • Total State.
    Methods in Molecular Biology,1064-3745 ; 1093
  • Contents Note
    Chromatin Immunoprecipitation Assay of Piwi in Drosophila -- Drosophila Germline Stem Cell for in vitro Analyses of PIWI-Mediated RNAi -- RNAi and Overexpression of Genes in Ovarian Somatic Cells -- Making piRNAs in vitro -- A Framework for piRNA Cluster Manipulation -- Mass Spectrometric Analysis of 3??End Methylation of piRNAs -- HITS-CLIP (CLIP-Seq) for Mouse Piwi Proteins -- Analysis of DNA혻Methylation in Mouse Testes -- Analysis of Small RNA-Guided Endonuclease Activity in Endogenous Piwi Protein Complexes from Mouse Testes -- Small RNA Library Construction from Minute Biological Samples -- Analysis of sDMA Modifications of PIWI Proteins -- Analyses of piRNA-Mediated Transcriptional Transposon Silencing in Drosophila: Nuclear Run-On Assay on Ovaries -- Combined RNA/DNA Fluorescence in situ Hybridization on Whole-Mount Drosophila Ovaries -- Fast and Accurate Method to Purify Small Non-Coding RNAs from Drosophila Ovaries -- Isolation of Zebrafish Gonads for RNA Isolation -- Small RNA Library Construction for High-Throughput Sequencing -- Analysis of Piwi-Loaded Small RNAs in Tetrahymena -- Effective Gene Knockdown in the Drosophila Germline by Artificial miRNA-Mimicking siRNAs -- Isolation and Bioinformatics Analyses of Small RNAs Interacting with Germ-Cell Specific Argonaute in Rice.
  • Topical Term
    Human genetics.
    Human Genetics.
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9781627036931
  • ISBN
  • Language