CRISPR Methods and Protocols [electronic resource] :

  • Lundgren, Magnus. editor. , Charpentier, Emmanuelle. editor. , Fineran, Peter C. editor.
  • 2015
CRISPR Methods and Protocols [electronic resource] :
  • Data Type
  • Title/Author
    CRISPR[electronic resource] :Methods and Protocols / edited by Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran.
  • Author
    Lundgren, Magnus., editor., Charpentier, Emmanuelle., editor., Fineran, Peter C., editor.
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    XIV, 366 p. 63 illus., 37 illus. in color. : online resource.
  • Total State.
    Methods in Molecular Biology,1064-3745 ; 1311
  • Contents Note
    Investigating CRISPR RNA Biogenesis and Function using RNA-seq -- In vitro Co-reconstitution of Cas Protein Complexes -- Analysis of CRISPR pre-crRNA Cleavage -- Annotation and Classification of CRISPR-Cas Systems -- Computational Detection of CRISPR/crRNA Targets -- High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium -- Spacer-based Macroarrays for CRISPR Genotyping -- Analysis of crRNA using Liquid Chromatography Electrospray Ionisation Mass Spectrometry (LC ESI MS) -- Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection through CRISPR -- Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification Of Target Interference Motifs in Escherichia coli -- Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes -- Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus -- Procedures for Generating CRISPR Mutants With Novel Spacers Acquired from Viruses Or Plasmids -- Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition -- Using the CRISPR-Cas system to Positively Select Mutants In Genes Essential for its Functionl -- Analysis of Nuclease Activity of Cas1 Proteins Against Complex DNA Substrates -- Characterizing Metal-Dependent Nucleases of CRISPR-Cas prokaryotic Adaptive Immunity Systems -- Cas3 Nuclease-Helicase Activity Assays -- Chemical and Enzymatic Footprint Analyses of R-loop Formation by Cascade-crRNA Complex -- Creation and Analysis of a Virome: Using CRISPR Spacers -- Targeted Mutagenesis in Zebrafish using CRISPR RNA-guided Nucleases -- Precise Genome Editing of Drosophila with CRISPR RNA-guided Cas9 -- Targeted Transcriptional Repression in Bacteria using CRISPR Interference (CRISPRi).
  • Topical Term
    Gene expression.
    Gene Expression.
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9781493926862
  • ISBN
  • Language