High-Resolution Imaging of Cellular Proteins Methods and Protocols [electronic resource] :

  • Schwartzbach, Steven D. editor. , Skalli, Omar. editor. , Schikorski, Thomas. editor.
  • 2016
High-Resolution Imaging of Cellular Proteins Methods and Protocols [electronic resource] :
  • Data Type
  • Title/Author
    High-Resolution Imaging of Cellular Proteins[electronic resource] :Methods and Protocols / edited by Steven D. Schwartzbach, Omar Skalli, Thomas Schikorski.
  • Author
    Schwartzbach, Steven D., editor., Skalli, Omar., editor., Schikorski, Thomas., editor.
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    XIII, 366 p. 45 illus., 21 illus. in color. : online resource.
  • Total State.
    Methods in Molecular Biology,1064-3745 ; 1474
  • Contents Note
    Expression of Epitope-Tagged Proteins in Mammalian Cells in Culture -- Antibody Production with Synthetic Peptides -- Production and Purification of Polyclonal Antibodies -- Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab??2 and Streptavidin -- Helper Dependent Adenoviral Vectors and their use for Neuroscience Applications -- Localizing Proteins in Fixed Giardia Lamblia and Live Cultured Mammalian Cells by Confocal Fluorescence Microscopy -- Using Fluorescent Protein Fusions to Study Protein Subcellular Localization and Dynamics in Plant Cells -- Using FRAP Or FRAPA to Visualize the Movement of Fluorescently-Labeled Proteins or Cellular Organelles in Live Cultured Neurons Transformed with Adeno-Associated Viruses -- Bimolecular Fluorescence Complementation (BiFc) Analysis of Protein-Protein Interactions and Assessment of Subcellular Localization in Live Cells -- Viral Injection and Cranial Window Implantation for In Vivo Two-Photon Imaging -- Imaging Synaptic Vesicle Exocytosis-Endocytosis with pH Sensitive Fluorescent Proteins -- Immunogold Protein Localization on Grid-Glued Freeze-Fracture Replicas -- Pre-Embedding Double-Label Immunoelectron Microscopy of Chemically Fixed Tissue Culture Cells -- Immunoelectron Microscopy of Cryofixed and Freeze- Substituted Plant Tissues -- Immunoelectron Microscopy of Cryofixed Freeze Substituted Yeast Saccharomyces cerevisiae -- Pre-embedding Method of Electron Microscopy for Glycan Localization in Mammalian Tissues and Cells Using Lectin Probes -- Pre-Embedding Nanogold Silver and Gold Intensification -- Post-Embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based On Heat-Induced Antigen Retrieval -- Pre- and Post-Embedding Immunogold Labeling of Tissue Sections -- Immuno-Gold Labelling for Scanning Electron Microscopy -- Monitoring Synaptic Vesicle Protein Sorting with Enhanced Horseradish Peroxidase in the Electron Microscope -- Functional Nanoscale Imaging Of Synaptic Vesicle Cycling With Superfast Fixation.
  • Topical Term
    Medicine & Public Health.
    Imaging / Radiology.
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9781493963508
  • ISBN
  • Language