Methods in Mammary Gland Biology and Breast Cancer Research [electronic resource]

  • Ip, Margot M. editor. , Asch, Bonnie B. editor.
  • 2000
Methods in Mammary Gland Biology and Breast Cancer Research [electronic resource]
  • Data Type
  • Title/Author
    Methods in Mammary Gland Biology and Breast Cancer Research[electronic resource] / edited by Margot M. Ip, Bonnie B. Asch.
  • Author
    Ip, Margot M., editor., Asch, Bonnie B., editor.
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    XVI, 329 p. : online resource.
  • Contents Note
    I. In Vivo Model Systems -- 1 Mouse Models for Mammary Cancer -- 2 Methods for the Induction of Mammary Carcinogenesis in the Rat Using either 7,12-Dimethylbenz[?]anthracene or 1-Methyl-1-Nitrosourea -- 3 A Comparison of the Salient Features of Mouse, Rat, and Human Mammary Tumorigenesis -- 4 Xenograft Models of Human Breast Cancer Lines and of the MCF10AT Model of Human Premalignant, Proliferative Breast Disease -- 5 Implantation and Characterization of Human Breast Carcinomas in SCID Mice -- II. Special Techniques for In Vivo Studies -- 6 The Cleared Mammary Fat Pad and the Transplantation of Mammary Gland Morphological Structures and Cells -- 7 Preparing Mammary Gland Whole Mounts from Mice -- 8 Slow-Release Plastic Pellets (Elvax) for Localized In Situ Treatments of Mouse and Rat Mammary Tissue -- 9 Intramammary Delivery of Hormones, Growth Factors, and Cytokines -- 10 Hormonal Stimulation of the Mouse Mammary Gland -- III. In Vitro Model Systems -- 11 Collagen Gel Method for the Primary Culture of Mouse Mammary Epithelium -- 12 Chemical-Carcinogen-Induced Transformation of Primary Cultures of Mouse Mammary Epithelial Cells Grown inside Collagen Gels -- 13 Establishment of Mouse Mammary Cell Lines -- 14 Whole Organ Culture of the Mouse Mammary Gland -- 15 Working with the Mouse Mammary End Bud -- 16 Isolation and Culture of Normal Rat Mammary Epithelial Cells -- 17 Characterization of Normal Human Breast Epithelial Cell Subpopulations Isolated by Fluorescence-Activated Cell Sorting and Their Clonogenic Growth In Vitro -- 18 Isolation and Culture of Human Breast Cancer Cells from Primary Tumors and Metastases -- IV Molecular Analysis and Gene Transfer Techniques -- 19 mRNA In Situ Hybridization in the Mammary Gland -- 20 Application of In Situ PCR to Studies of the Mammary Gland -- 21 Transfection of Primary Mammary Epithelial Cells by Viral and Nonviral Methods -- 22 Direct Gene Transfer into the Mammary Epithelium In Situ Using Retroviral Vectors -- 23 Intraductal Injection into the Mouse Mammary Gland -- 24 Adenoviral and Transgenic Approaches for the Conditional Deletion of Genes from Mammary Tissue -- 25 Transplantation and Tissue Recombination Techniques to Study Mammary Gland Biology -- 26 Rescue of Mammary Epithelium of Early Lethal Phenotypes by Embryonic Mammary Gland Transplantation as Exemplified with Insulin Receptor Null Mice.
  • Topical Term
    Cancer research.
    Cancer Research.
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9781461369271
  • ISBN
  • Language