Neuropeptide Protocols [electronic resource]

  • Irvine, G. Brent. editor. , Williams, Carvell H. editor.
  • 1997
Neuropeptide Protocols [electronic resource]
  • Data Type
  • Title/Author
    Neuropeptide Protocols[electronic resource] / edited by G. Brent Irvine, Carvell H. Williams.
  • Author
    Irvine, G. Brent., editor., Williams, Carvell H., editor.
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    XIII, 386 p. 22 illus. : online resource.
  • Total State.
    Methods in Molecular Biology,1064-3745 ; 73
  • Contents Note
    Preparation of Neuropeptide-Containing Fractions from Biological Materials -- Purification of Extracted Peptides for Structural Analysis -- Amino Acid Sequencing of Neuropeptides -- Neuropeptide Gene Identification Using the Polymerase Chain Reaction -- Solid-Phase Synthesis of Neuropeptides by Fmoc Strategies -- Incorporation of Stable Pseudopeptide Bonds -- Synthesis of Conformationally Restricted Peptides -- Purification of Synthetic Peptides by High Performance Liquid Chromatography -- Molecular Weight Estimation for Neuropeptides Using Size-Exclusion High Performance Liquid Chromatography -- Molecular Weight Determinations Using Polyacrylamide Gel Electrophoresis with Tris-Tricine Buffers -- Determination of Neuropeptides by Capillary Electrophoresis -- Characterization of Neuropeptide Processing by Fast Atom Bombardment Mass Spectrometry -- Analysis of Neuropeptides by Size-Exclusion HPLC Linked to Electrospray Ionization Mass Spectrometry -- Identification of Peptides by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) and Direct Analysis of the Laterobuccal Nerve from the Pond Snail Lymnaea stagnalis -- Use of Circular Dichroism to Determine Secondary Structure of Neuropeptides -- 1H Nuclear Magnetic Resonance (NMR) in the Elucidation of Peptide Structure -- The Study of Membrane- or Receptor-Bound Neuropeptides by NMR -- Molecular Modeling of Neuropeptides -- Tritium Labeling of Neuropeptides -- The Use of IODO-GEN for Preparing 125I-Labeled Peptides and Their Purification by Reversed-Phase High Performance Liquid Chromatography -- Production of Antisera Using Peptide Conjugates -- Radioimmunoassay -- Enzyme-Linked Immunosorbent Assay of Peptides -- Sample Preparation for Peptide Immunocytochemistry -- lmmunocytochemical Methods for Regulatory Peptides -- Ultrastructural Localization of Peptides Using Immunogold Labeling -- Preparation of a Membrane Fraction for Receptor Studies and Solubilization of Receptor Proteins with Retention of Biological Activity -- Radioligand Binding Using 125Labeled Peptides -- Analysis of Data from ?쏞old Saturation??Radioligand Binding Experiments -- Organ/Tissue Preparations for the Assessment of Agonist/Antagonist Activity -- Measurement of Efflux Rates from Brain to Blood -- Assay of Neuropeptidases Using Fluorogenic Substrates -- Characterization of Neuropeptidases Using Inhibitors.
  • Topical Term
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9780896033993
  • ISBN
  • Language