Stem cells and aging : methods and protocols [electronic resource]

  • Turksen, Kursad
  • Humana Press
  • 2020
Stem cells and aging : methods and protocols [electronic resource]


This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but novices in the ever-developing stem cell field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Table of Content

Assessing Muscle Stem Cell Clonal Complexity During Aging / Matthew T. Tierney, Michael J. Stec, Alessandra Sacco
Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies / Eusebio Perdiguero, Victoria Moiseeva, Pura Muñoz-Cánoves
Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle / Sören S. Hüttner, Hellen E. Ahrens, Manuel Schmidt, Henriette Henze, Marie Juliane Jung, Svenja C. Schüler et al.
Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue / Meenakshi Gaur, Marek Dobke, Victoria V. Lunyak
Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures / Stéphanie Nadeau, Anastasia Cheng, Inés Colmegna, Francis Rodier
Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells / Courtney R. Ogando, Gilda A. Barabino, Yueh-Hsun Kevin Yang
Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis / Klemen Čamernik, Janja Marc, Janja Zupan
Complete Assessment of Multilineage Differentiation Potential of Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cells / Klemen Čamernik, Janja Zupan
Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis / Janja Zupan
3D-Embedded Cell Cultures to Study Tendon Biology / Renate Gehwolf, Gabriel Spitzer, Andrea Wagner, Christine Lehner, Nadja Weissenbacher, Herbert Tempfer et al.
Targeted, Amplicon-Based, Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis / Brooke Snetsinger, Christina K. Ferrone, Michael J. Rauh
Column-Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants / Sherin Saheera, Renuka R. Nair
Histological Assessment of Cre-loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreERT2/Rosa26YFP Mice / Saad Omais, Nour N. Halaby, Karl John Habashy, Carine Jaafar, Anthony T. Bejjani, Noël Ghanem
Infrared Spectroscopy and Imaging in Stem Cells and Aging Research / Ceren Aksoy, Feride Severcan
Use of U-STELA for Accurate Measurement of Extremely Short Telomeres / Nedime Serakinci, Huseyin Cagsin, Merdiye Mavis
Surface Antigen-Based Identification of In Vitro Expanded Skeletal Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry / Klemen Čamernik, Janja Zupan
Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry / Rasmus Jentoft Boutrup
Methods for Detection of Autophagy in Mammalian Cells / Bindu Singh, Sangeeta Bhaskar
Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells / Yohei Hayashi, Yasuhisa Matsui
Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer / Dan-Ya Wu, Xia Zhang, Yi-Liang Miao
Generation of Transplantable Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD) / Harshini Surendran, Reena J. Rathod, Rajarshi Pal
Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury / Jea-Young Lee, Roger Lin, Hung Nguyen, M. Grant Liska, Trenton Lippert, Yuji Kaneko et al.
3D Age-Specific Mortality Trajectory: A Survival Analysis Protocol / Yuhui Lin
Isolation, Expansion, and Characterization of Wharton’s Jelly-Derived Mesenchymal Stromal Cell: Method to Identify Functional Passages for Experiments / Shuh-Wen Aung, Noor Hayaty Abu Kasim, Thamil Selvee Ramasamy
CRISPR Base Editing in Induced Pluripotent Stem Cells / Ya-Ju Chang, Christine L. Xu, Xuan Cui, Alexander G. Bassuk, Vinit B. Mahajan, Yi-Ting Tsai et al.