E-Book

X-Chromosome Inactivation: Methods and Protocols [electronic resource]

  • Sado, Takashi. editor.
  • Springer
  • 2018
X-Chromosome Inactivation: Methods and Protocols [electronic resource]
  • Data Type
    Book
  • Title/Author
    X-Chromosome Inactivation:Methods and Protocols[electronic resource] / edited by Takashi Sado.
  • Publication
    New York, NY : Springer, 2018.
  • Author
    Sado, Takashi., editor., edt, http://id.loc.gov/vocabulary/relators/edt
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    XI, 251 p. 53 illus., 43 illus. in color. : online resource.
  • Total State.
    Methods in Molecular Biology,1064-3745 ; 1861
  • Contents Note
    Screening for Factors Involved in X Chromosome Inactivation using Haploid ESCs -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Unbiased Genetic Screen to Identify Factors Involved in X-chromosome Inactivation Using a Pooled Bar-coded shRNA Library -- Manipulation of Xist Imprinting in Mouse Pre-implantation Embryos -- Somatic Cell Nuclear Transfer in Mice ??Basic Protocol and Its Modification for Correcting X Chromosome Inactivation Status -- Live Imaging of Xist RNA -- Live Imaging of X-chromosome Inactivation and Reactivation Kinetics -- Visualizing the Dynamics of Inactive X chromosomes in Living Cells Using Antibody-based Fluorescent Probes -- Visualization of Hidden Epitopes at the Inactive X Chromosome -- Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation -- Simultaneous RNA-DNA FISH in Mouse Pre-implantation Embryos -- Combined Immunofluorescence, RNA FISH and DNA FISH in Pre-implantation Mouse Embryos -- RNA-FISH and Immunofluorescence of Mouse Pre- and Post-implantation Embryos -- Experimental Analysis of Imprinted Mouse X-chromosome Inactivation -- X-chromosome Inactivation and Escape from X inactivation in Mouse -- Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles.
  • Topical Term
    Human genetics.
    Human Genetics. http://scigraph.springernature.com/things/product-market-codes/B12008
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9781493987658
  • ISBN
    9781493987665
  • Language
    English

Abstract

This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.