E-Book

Yeast Cytokinesis Methods and Protocols [electronic resource] :

  • Sanchez-Diaz, Alberto. editor. , Perez, Pilar. editor.
  • 2016
Yeast Cytokinesis Methods and Protocols [electronic resource] :
  • Data Type
    Book
  • Title/Author
    Yeast Cytokinesis[electronic resource] :Methods and Protocols / edited by Alberto Sanchez-Diaz, Pilar Perez.
  • Edition
    1st ed. 2016.
  • Author
    Sanchez-Diaz, Alberto., editor., Perez, Pilar., editor.
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    XI, 395 p. 83 illus., 52 illus. in color. : online resource.
  • Total State.
    Methods in Molecular Biology,1064-3745 ; 1369
  • Contents Note
    Time-lapse Fluorescence Microscopy Of Budding Yeast Cells -- Real-time Visualization and Quantification of Contractile Ring Proteins in Single Living Cells -- Fluorescence Recovery After Photo-bleaching (FRAP) and Fluorescence Loss in Photo-bleaching (FLIP) Experiments to Study Protein Dynamics during Budding Yeast Cell Division -- High-Speed Super Resolution Imaging of Live Fission Yeast Cells -- Monitoring Chitin Deposition during Septum Assembly in Budding Yeast -- Imaging Septum Formation by Fluorescence Microscopy -- Visualization of Cytokinesis Events in Budding Yeast by Transmission Electron Microscopy -- Visualization of Fission Yeast Cells By Transmission Electron Microscopy -- Characterization of Septin Ultrastructure in Budding Yeast Using Electron Tomography -- Isolation of Cytokinetic Actomyosin Rings from Saccharomyces cerevisiae and Schizosaccharomyces pombe -- Measurements of Myosin-II motor Activity during Cytokinesis In Fission Yeast -- In Vitro Biochemical Characterization of Cytokinesis Actin Binding Proteins -- Characterization of Cytokinetic F-BARs and other Membrane Binding Proteins -- Analysis of Three-Dimensional Structures of혻Exocyst혻Components -- Analysis of Rho-GTPase Activity during Budding-yeast Cytokinesis -- Detection of Phosphorylation Status of Cytokinetic Components -- Studying Protein-Protein Interactions in Budding Yeast Using Co-Immunoprecipitation -- Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening Of Suppressor Genes -- Synchronization of the Budding Yeast Saccharomyces cerevisiae -- Fission Yeast Cell Cycle Synchronization Methods -- A Review of Fluorescent Proteins for Use in Yeast -- Visualisation and Image Analysis of Yeast Cells -- Toolbox for Protein Structure Prediction -- From Structure to Function. A Comprehensive Compendium of Tools to Unveil Protein Domains and Understand their Role in Cytokinesis.
  • Topical Term
    Life sciences.
    Cell biology.
    Life Sciences.
    Cell Biology.
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9781493931446
  • ISBN
    9781493931453
  • Language
    English