Nanotoxicity : methods and protocols [electronic resource]
- Data Type
- Nanotoxicity :methods and protocols[electronic resource] / edited by Qunwei Zhang.
- New York, NY : Springer, 2019.
- Zhang, Qunwei
- Phy Desc.
- 1 online resource.
- Total State.
- Methods in Molecular Biology1064-3745 ; 1894
- 9781493989157, 9781493989164
This volume explores the latest techniques used to investigate nanotoxicity. The protocols covered in this book range from routine nanotoxicity assays to genomic and proteomic approaches in vivo and in vitro. The chapters discuss specific topics such as preparation and utilization of 3D human liver microtissue models; assessment of ovarian granulosa cells with exposure to nanoparticles; DNA methylation analysis; evaluation of genotoxicity of nanoparticles in mouse models; and studying nanotoxicity using embryonic zebrafish. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Nanotoxicity: Methods and Protocols is a valuable resource for researchers and postgraduate students in the fields of toxicology, environmental science, pharmacological sciences, and clinical medicine. This volume also serves as a starting point for researchers interested in learning more about this developing field.
Table of Content
Methods for assessing mast cell responses to engineered nanomaterial exposure / Nasser B. Alsaleh and Jared M. Brown
Preparation and u tilization of a 3D human liver microtissue model for nanotoxicological assessment / Ali Kermanizadeh
Deriving neural cells from pluripotent stem cells for nanotoxicity testing / Yiling Hong, Nymph Chan, and Aynun N. Begum
Methods for evaluation of ovarian granulosa cells with exposure to nanoparticles / Jin Liu and Wenchang Zhang
In vitro approaches for assessing the genotoxicity of nanomaterials / Maria Dusinska, Espen Mariussen, Elise Runde´n-Pran, Alexandra Misci Hudecova, Elisabeth Elje, Alena Kazimirova, Naouale El Yamani, Nils Dommershausen, Julian Tharmann, Dagmar Fieblinger, Frank Herzberg, Andreas Luch, and Andrea Haase
Measurement of nanoparticle-induced mitochondrial membrane potential alterations / Salik Hussain
Application of gelatin zymography in nanotoxicity research / Yue Zhang, Rong Wan, Qunwei Zhang, and Yiqun Mo
Determination of phosphorylated histone H2AX in nanoparticle-induced genotoxic studies / Rong Wan, Yiqun Mo, Ruirui Tong, Meiqin Gao, and Qunwei Zhang
Analysis of nanomaterial toxicity by western blot / Gao Long, Yiqun Mo, Qunwei Zhang, and Mizu Jiang
Quantification of mitochondrial genome transcription in male reproductive cells by real-time PCR / Cheng Xu, Qian Liu, Jin Xu, and Aihua Gu
DNA methylation analysis / Lingfang Feng and Jianlin Lou
Evaluation of Nrf2 with exposure to nanoparticles / Fuli Zheng and Huangyuan Li
Application of electron microscopes in nanotoxicity assessment / Junzhe Zhang, Xiao He, and Michael T. Tseng
Metabolic fingerprinting of bacteria exposed to nanomaterials, using online databases, NMR, and high-resolution mass spectrometry / Theodoros G. Chatzimitakos and Constantine D. Stalikas
Methods to quantify nanomaterial association with, and distribution across, the blood-brain barrier in vivo / Robert A. Yokel
Evaluation of genotoxicity of nanoparticles in mouse models / Sameera Nallanthighal and Ramune Reliene
Evaluation of pulmonary toxicity of nanoparticles by bronchoalveolar lavage / Yiqun Mo, Yue Zhang, and Qunwei Zhang
Pulmonary function testing in animals / Gary W. Hoyle, Connie F. Schlueter, and Sadiatu Musah
Nanotoxicity assessment using embryonic zebrafish / Eduard Dumitrescu, Kenneth Wallace, and Silvana Andreescu
Application of Daphnia magna for nanoecotoxicity study / Zhizhen Xu, Yingying Liu, and Yuqian Wang
Overview of methods of nanoparticle exposure assessment / Peng Zhao and Yuanbao Zhang.