E-Book

Neural stem cells: methods and protocols [electronic resource]

  • Daadi, Marcel M.
  • Springer
  • 2019
Neural stem cells: methods and protocols [electronic resource]
  • Data Type
    Book
  • Title/Author
    Neural stem cells:methods and protocols[electronic resource] / edited by Marcel M. Daadi.
  • Publication
    New York, NY : Springer, 2019.
  • Author
    Daadi, Marcel M.
  • Phy Desc.
    1 online resource.
  • Total State.
    Methods in Molecular Biology1064-3745 ; 1919
  • Topical Term
    Neural stem cells -- Laboratory manuals
    Neural Stem Cells.
  • ISBN
    9781493990054, 9781493990078
  • Language
    English

Abstract

This volume details various technological advances to isolate, perpetuate, and characterize neural stem cells from various sources and formulates them for the delivery into animal models of neurological disorders. Chapters guide the reader through methods and protocols on definition and isolation of neural stem cells, characterization and differentiation, standards for defining cell populations, and In vivo approaches in animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Table of Content

Generation of Neural Stem Cells from Induced Pluripotent Stem Cells
Making NSC and Neurons from Patient-Derived Tissue Samples
Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury
Derivation of Neural Stem Cells from Human Parthenogenetic Stem Cells
Chemically Defined Neural Conversion of Human Pluripotent Stem Cells
In Vitro Functional Characterization of Human Neurons and Astrocytes Using Calcium Imaging and Electrophysiology
Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons
Midbrain Dopaminergic Neurons Differentiated from Human-Induced Pluripotent Stem Cells
Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene
Single-Cell Library Preparation of iPSC-Derived Neural Stem Cells
Bioinformatics Analysis of Single-Cell RNA-Seq Raw Data from iPSC-Derived Neural Stem Cells
Assay for Assessing Mitochondrial Function in iPSC-Derived Neural Stem Cells and Dopaminergic Neurons
Reference Transcriptome for Deriving Marmoset Induced Pluripotent Stem Cells
Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach
Isolation and Differentiation of Self-Renewable Neural Stem Cells from Marmoset-Induced Pluripotent Stem Cells
Lentiviral Infection of Mouse Bone Marrow Cells for Hematopoietic Stem Cell Transplantation
Central and Peripheral Secondary Cell Death Processes after Transient Global Ischemia in Nonhuman Primate Cerebellum and Heart
Method for Stimulation of Hippocampal Neurogenesis by Transient Microneedle Insertion