RNA Chaperones: Methods and Protocols [electronic resource]

  • Heise, Tilman. editor.
  • Humana
  • 2020
RNA Chaperones: Methods and Protocols [electronic resource]
  • Data Type
  • Title/Author
    RNA Chaperones:Methods and Protocols[electronic resource] / edited by Tilman Heise.
  • Edition
    1st ed.
  • Publication
    New York, NY : Humana, 2020.
  • Author
    Heise, Tilman., editor., edt, http://id.loc.gov/vocabulary/relators/edt
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    XI, 314 p. 78 illus., 70 illus. in color. : online resource.
  • Total State.
    Methods in Molecular Biology,1064-3745 ; 2106
  • Contents Note
    Biochemical Methods for the Study of the FinO Family of Bacterial RNA Chaperones -- Quantitative Analysis of RNA Chaperone Activity by Native Gel Electrophoresis and Fluorescence Spectroscopy -- Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity -- Specific Nucleic Acid Chaperone Activity of HIV-1 Nucleocapsid Protein Deduced from Hairpin Unfolding -- Real-Time Fluorescence-Based Approaches to Disentangle Mechanisms of a Protein?셲 RNA Chaperone Activity -- Use of tRNA-Mediated Suppression to Assess RNA Chaperone Function -- Salt-Dependent Modulation of the RNA Chaperone Activity of RNA-Binding Protein La -- Kinetic and Thermodynamic Analyses of RNA?밣rotein Interactions -- Detection of MicroRNAs Released from Argonautes -- Mapping the RNA Chaperone Activity of the T. brucei Editosome Using SHAPE-Chemical Probing -- RNA Remodeling by RNA Chaperones Monitored by RNA Structure Probing -- In Vivo RNA Chemical Footprinting Analysis in Archaea -- RNA Structure Analysis by Chemical Probing with DMS and CMCT -- Disordered RNA Binding Region Prediction with DisoRDPbind -- Delivering Molecular Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of Single RNA Transcripts and Genomic Loci -- Site-Specific Dual-Color Labeling of Long RNAs -- Single-Molecule FRET Assay for Studying Cotranscriptional RNA Folding -- Characterizing Complex Nucleic Acid Interactions of LINE1 ORF1p by Single Molecule Force Spectroscopy -- Isolation and Analysis of Bacterial Ribosomes through Sucrose Gradient Ultracentrifugation.
  • Topical Term
    Human genetics.
    Human Genetics. http://scigraph.springernature.com/things/product-market-codes/B12008
    Protein Science. http://scigraph.springernature.com/things/product-market-codes/L14040
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9781071602300
  • ISBN
  • Language


This book provides a wide spectrum of methods to study RNA chaperones in vitro, at the single molecule level, and protocols useful for cell-based assays. Beginning with a section on a number of bacterial proteins for study, the volume also explores proteins from eukaryotic cells and how to delve into the complex interactions between RNA chaperones and the folding and unfolding of proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, RNA Chaperones: Methods and Protocols serves as an ideal guide for scientists and students interested in RNA biology and RNA chaperones. Chapter 3 is available Open Access under a CC-BY 4.0 license via link.springer.com.