E-Book

Nuclear Reprogramming Methods and Protocols [electronic resource] :

  • Pells, Steve. editor.
  • 2006
Nuclear Reprogramming Methods and Protocols [electronic resource] :
  • Data Type
    Book
  • Title/Author
    Nuclear Reprogramming[electronic resource] :Methods and Protocols / edited by Steve Pells.
  • Author
    Pells, Steve., editor.
  • Authors
    SpringerLink (Online service)
  • Phy Desc.
    XIV, 334 p. : online resource.
  • Total State.
    Methods in Molecular Biology,1064-3745 ; 325
  • Contents Note
    Nuclear Transplantation in Xenopus -- Nuclear Transfer in Sheep -- Protocols for Nuclear Transfer in Mice -- Isolation of Stromal Stem Cells From Human Adipose Tissue -- Nuclear Reprogramming by Cell Fusion -- Polyethylene Glycol-Mediated Cell Fusion -- Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells -- Quantification of Cell Fusion by Flow Cytometry -- Modulation of Cell Fate Using Nuclear and Cytoplasmic Extracts -- Transgenic Systems in Nuclear Reprogramming -- Using Immunofluorescence to Observe Methylation Changes in Mammalian Preimplantation Embryos -- Observing S-Phase Dynamics of Histone Modifications With Fluorescently Labeled Antibodies -- Quantitative Analysis of Telomerase Activity and Telomere Length in Domestic Animal Clones -- Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells -- Staining Embryonic Stem Cells Using Monoclonal Antibodies to Stage-Specific Embryonic Antigens -- Analysis of the Nucleolar Compartment of the Nucleus as an Indicator of Nuclear Reprogramming After Nuclear Transfer -- Methylation-Sensitive Polymerase Chain Reaction -- Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis -- Chromatin Immunoprecipitation Assay for Mammalian Tissues -- Histone Modifications and Transcription Factor Binding on Chromatin: ChIP?밣CR Assays -- In Vivo Genomic Footprinting Using LM?밣CR Methods -- Analyzing Histone Modification Using Crosslinked Chromatin Treated With Micrococcal Nuclease.
  • Topical Term
    Life sciences.
    Biochemistry.
    Cell biology.
    Life Sciences.
    Biochemistry, general.
    Cell Biology.
  • Host Item
    Springer eBooks
  • Additional Item
    Printed edition: 9781588293794
  • ISBN
    9781597450058
  • Language
    English