XVI, 547 p. 120 illus., 89 illus. in color. : online resource.
총서명
Methods in Molecular Biology,1064-3745 ; 1427
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Helios짰 Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: Recent Updates -- Tol2-Mediated Delivery of miRNAs to the Chicken Otocyst using Plasmid Electroporation -- A Rapid, Cost-Effective Method of Recombinant Adeno-Associated Virus Preparation Enables Efficient Gene Transfer to the Developing Mouse Inner Ear -- Generation of Noninvasive, Quantifiable, Orthotopic Animal Models for NF2-Associated Schwannoma and Meningioma -- RNA Extraction from Xenopus Auditory and Vestibular Organs for Molecular Cloning and Expression Profiling with RNA-Seq and Microarrays -- Yeast Two-Hybrid Screening to Test for Protein-Protein Interactions in the Auditory System -- Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research -- Protein Quantification of the Developing Cochlea by Mass Spectrometry -- Ultrastructural Identification and Colocalization of Interacting Proteins in the Murine Cochlea by Post-Embedding Immunogold Transmission Electron Microscopy -- Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Inner-Ear Proteins -- The Single-Molecule Approach to Membrane Protein Stoichiometry -- Visualization of Live Cochlear Stereocilia at a Nanoscale Resolution Using Hopping Probe Ion Conductance Microscopy -- Design and Construction of a Cost-Effective Spinning Disk System for Live Imaging of Inner Ear Tissue -- Neuroanatomical Tracing Techniques: History, State of the Art, and Future Developments -- Detection of Excitation and Inhibition in the Auditory System Using Fluorescence Immunohistochemistry and High Resolution Fluorescence Microscopy -- Rapid and Semi-Automated Extraction of Neuronal Cell Bodies and Nuclei from Electron Microscopy Image Stacks.-Organotypic Culture of the Mouse Cochlea from Embryonic Day 12 to the Neonate -- Quantifying Spiral Ganglion Neurite and Schwann Behavior on Micropatterned Polymer Substrates -- The Use of Human Wharton?셲 Jelly Cells for Cochlear Tissue Engineering -- Nanotechnology in Auditory Research: Membrane Electromechanics in Hearing -- Overview of Nanoparticle Based Delivery for Treatment of Inner Ear Disorders -- Development of Cell-based High-Throughput Chemical Screens for Protection Against Cisplatin-induced Ototoxicity.-Profiling Specific Inner Ear Cell Types Using Cell Sorting Techniques -- Optical Coherence Tomography to Measure Sound-Induced Motions Within the Mouse Organ of Corti In Vivo -- Method for Dissecting the Auditory Epithelium (Basilar Papilla) in Developing Chick Embryos -- Whole Cell Patch Clamp Recording of Mouse and Rat Inner Hair Cells in the Intact Organ of Corti -- Glass Probe Stimulation of Hair Cell Stereocilia -- A Walkthrough of Nonlinear Capacitance Measurement of Outer Hair Cells -- In Vitro Functional Assessment of Adult Spiral Ganglion Neurons (SGNs) -- Mapping Auditory Circuits with Photostimulation of Caged Glutamate. .